ProGP378 (Putative membrane protein)

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ProGP ID ProGP378 (Putative membrane protein)
Validation Status Characterized
Organism Information
Organism NameCampylobacter jejuni HB93-13
Domain Bacteria
Classification Family: Campylobacteraceae
Order: "Campylobacterales"
Class: "Epsilonproteobacteria"
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 192222
Genome Information
GenBank NC_002163.1.
EMBL AL111168
Organism Additional Information Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity.
Gene Information
Gene Namecj0648
NCBI Gene ID 904975.
GenBank Gene Sequence NC_002163.1.
Protein Information
Protein NamePutative membrane protein
UniProtKB/SwissProt ID Q0PAM0
NCBI RefSeq WP_002855039.1
EMBL-CDSCAL34793.1.
UniProtKB Sequence >tr|Q0PAM0|Q0PAM0_CAMJE Putative membrane protein OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0648 PE=4 SV=1 MAIKIFGILIALFTITFTILSLQDPYSLNLQTNALNFKNIEAKNLKAYESNTSIIKAYYK ANSWVRYADRDEFNDFITLNLDFNLSANRLEFFNKDMSKVLFEGNVTYIGSNNVKIISQE VEYQPKDKILHTNTNFKALINGSIINGNTLNYDVKNKILNIQGVNAWLQDK
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN51
Glycosite(s) Annotated Protein Sequence >tr|Q0PAM0|Q0PAM0_CAMJE Putative membrane protein OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0648 PE=4 SV=1 MAIKIFGILIALFTITFTILSLQDPYSLNLQTNALNFKNIEAKNLKAYESN*(51)TSIIKAYYK ANSWVRYADRDEFNDFITLNLDFNLSANRLEFFNKDMSKVLFEGNVTYIGSNNVKIISQE VEYQPKDKILHTNTNFKALINGSIINGNTLNYDVKNKILNIQGVNAWLQDK
Sequence Around Glycosites (21 AA) EAKNLKAYESNTSIIKAYYKA
Technique(s) used for Glycosylation DetectionZIC-HILIC enrichment
Technique(s) used for Glycosylated Residue(s) Detection Reversed Phase LC-Tandem CID/HCD-MS
Glycan Information
Glycan Annotation Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glc β1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac- β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose)
BCSDB ID20059
Technique(s) used for Glycan Identification Reversed Phase LC-Tandem CID/HCD-MS
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.10
Year of Validation 2011
ReferenceScott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ. (2011) Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Mol Cell Proteomics., 10(2):M000031-MCP201. [PubMed: 20360033]
AuthorScott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ
Research GroupSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
Corresponding Author Cordwell SJ
ContactSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia