ProGP168

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ProGP ID ProGP168
Validation Status Uncharacterized
Organism Information
Organism NameBacillus cereus ATCC 10876
Domain Bacteria
Classification Family: Bacillaceae
Order: Bacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI) 1396
Genome Sequence(s)
GenBank NZ_MNKZ01000327.1. 
EMBL AF246294 
Protein Information
Protein NameBclA (collagen-like protein)
Subcellular LocationSurface
Function 1)205-, 70-, and 30-kDa glycoproteins are present in the exosporium which is a transparent and loose covering of the spore.2) BclA is the major constituent of the hairy surface of exosporium, the resistant and flexible paracrystalline layer of spores. The exosporium plays a role in spore adhesion and virulence. The trimerized glycoprotein BclA is attached to the basal layer through an N-terminal domain.
Glycosylation Status
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionGlycoprotein detection kit
Glycan Information
Glycan Annotation Sugars identified include glucose, rhamnose, ribose, and glucosamine (amino sugar). 3.8% total amino sugar content determined.
In Bacillus cereus strain T, carbohydrates were determined using differential anthrone method, orcinol method, and aniline phthalic acid and ammoniacal silver nitrate reactions after descending paper chromatography.
Literature
Year of Identification1999
Year of Identification Month Wise1999.1
ReferenceTodd, S.J., Moir, A.J., Johnson, M.J. and Moir, A. (2003) Genes of Bacillus cereus and Bacillus anthracis encoding proteins of the exosporium. J Bacteriol, 185, 3373-3378. [PubMed: 12754235 ]
AuthorTodd, S.J., Moir, A.J., Johnson, M.J. Moir, A.
Research GroupDepartment of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom.
Corresponding Author Moir, A.
ContactDepartment of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom.
ReferenceCharlton, S., Moir, A.J., Baillie, L. and Moir, A. (1999) Characterization of the exosporium of Bacillus cereus. J Appl Microbiol, 87, 241-245. [PubMed: 10475957]
AuthorCharlton, S., Moir, A.J., Baillie, L. Moir, A.
Research GroupDepartment of Molecular Biology and Biotechnology, University of Sheffield, UK.
Corresponding Author Moir, A.
ContactDepartment of Molecular Biology and Biotechnology, University of Sheffield, UK.
ReferenceMatz,L.L., Beaman,T.C.andGerhardt,P. (1970) Chemical composition of exosporium from spores of Bacillus cereus. J Bacteriol, 101, 196-201.
AuthorMatz, L.L., Beaman, T.C. Gerhardt, P.
Corresponding Author Gerhardt, P.