ProGP392

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ProGP ID ProGP392
Validation Status Characterized
Organism Information
Organism NameCampylobacter jejuni HB93-13
Domain Bacteria
Classification Family: Campylobacteraceae
Order: "Campylobacterales"
Class: "Epsilonproteobacteria"
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 192222
Genome Sequence(s)
GenBank NC_002163.1. 
EMBL AL111168 
Gene Information
Gene NamecmeE
NCBI Gene ID 905324. 
GenBank Gene Sequence NC_002163.1. 
Protein Information
Protein NameCj1032 (Membrane fusion component multidrug efflux system)
UniProtKB/SwissProt ID Q0P9M0
NCBI RefSeq WP_002858463.1
EMBL-CDS CAL35150.1.
UniProtKB Sequence >tr|Q0P9M0|Q0P9M0_CAMJE Membrane fusion component of efflux system (Mutlidrug efflux system CmeDEF) OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=cmeE PE=3 SV=1 MKKILFLLLVFNCAFGEEIYASFNVEASKQSKLALESIGLVQKIPVEIGQKVSKGELLLA LDQESEKIALQNAQNSYQLALVEYENTKSRMQKIKAVENVIDKQSYEDMKAKFDAANLNL NKAKINIAYYKNIMAKKELRAPYDAIIANKFIQVGEGVGGVAQPLIEIFSYPQSKLILSF DEKYKDKVRLGDDFFYKIDQNGTELKGKINLIYPSIEVKTRKIYAEVQTTNLTPGLFGEG RIITKD
Sequence length 246 AA
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN201
Glycosite(s) Annotated Protein Sequence >tr|Q0P9M0|Q0P9M0_CAMJE Membrane fusion component of efflux system (Mutlidrug efflux system CmeDEF) OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=cmeE PE=3 SV=1 MKKILFLLLVFNCAFGEEIYASFNVEASKQSKLALESIGLVQKIPVEIGQKVSKGELLLA LDQESEKIALQNAQNSYQLALVEYENTKSRMQKIKAVENVIDKQSYEDMKAKFDAANLNL NKAKINIAYYKNIMAKKELRAPYDAIIANKFIQVGEGVGGVAQPLIEIFSYPQSKLILSF DEKYKDKVRLGDDFFYKIDQN*(201)GTELKGKINLIYPSIEVKTRKIYAEVQTTNLTPGLFGEG RIITKD
Sequence Around Glycosites (21 AA) GDDFFYKIDQNGTELKGKINL
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionSoybean Lectin Affinity Enrichment and ZIC-HILIC enrichment
Technique(s) used for Glycosylated Residue(s) Detection Reversed Phase LC-Tandem CID/HCD-MS, CID/ETD-MS and ESI (CID)-MS/MS after enrichment using graphite microcolumns
Glycan Information
Glycan Annotation Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glc β1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac- β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose)
BCSDB ID20059
Technique(s) used for Glycan Identification Reversed Phase LC-Tandem CID/HCD-MS
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.10
Year of Validation 2011
ReferenceScott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ. (201Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Mol Cell Proteomics. 2011 Feb;10(2):M000031-MCP201.
AuthorScott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ
Research GroupSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
Corresponding Author Cordwell SJ
ContactSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia