ProGP661 (Eight cysteine cluster domain containing protein)
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ProGP ID | ProGP661 (Eight cysteine cluster domain containing protein) |
Validation Status | Uncharacterized |
Organism Information | |
Organism Name | Pyrobaculum calidifontis JCM 11548 |
Domain | Archaea |
Classification | Phylum : Crenarchaeota Class : Thermoprotei Orders : Thermoproteales Family : Thermoproteaceae Genus : Pyrobaculum Species : calidifontis Strain : JCM 11548 |
Taxonomic ID (NCBI) | 410359 |
Genome Information | |
GenBank | CP000561.1 |
EMBL | CP000561 |
Organism Additional Information | Pyrobaculum calidifontis is a hyperthermophilic archaeon that belongs to the phylum Crenarchaeota. In contrast to the phylum Euryarchaeota, only the N-glycan structure of the genus Sulfolobus is known in Crenarchaeota. |
Gene Information | |
Gene Name | Pcal_1255 |
Protein Information | |
Protein Name | Eight-cysteine-cluster domain-containing protein |
UniProtKB/SwissProt ID | A3MVL2 |
NCBI RefSeq | ABO08679.1. |
EMBL-CDS | ABO08679.1 |
UniProtKB Sequence | >tr|A3MVL2|A3MVL2_PYRCJ Uncharacterized protein OS=Pyrobaculum calidifontis (strain JCM 11548 / VA1) GN=Pcal_1255 PE=4 SV=1 MVVKFVLALVLVALVVWSFVELGGGCCTLRQGFCYGSLCGYVLNFTSCQSFGNYTPYAEV KAVDGAVLVSGRVVPNCCSEVLKAVVSESGGVLFIYLLEADLDGVLCKCVCPRDFNLSTT WSGREVSVLWVVFHGGALASAEVLYTTFCGVSTYGECKSDADCVRDGCSGEVCRSVHEEA TPTPCVWRECFDAGRYRLKCGCVNGRCQWTYAG |
Sequence length | 213 AA |
Function | Hypothetical protein |
Glycosylation Status | |
Glycosylation Type | N- (Asn) linked |
Technique(s) used for Glycosylation Detection | ConA(Concanavalin A) lectin affinity chromatography and detected by periodic acid-Schiff (PAS) staining and Coomassie Brilliant Blue (CBB) staining on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gels, RP-HPLC |
Technique(s) used for Glycosylated Residue(s) Detection | Normal-phase-HPLC-MS/MS analysis, LC-ESI-MS/MS analysis |
Glycan Information | |
Glycan Annotation | Pentasaccharide (α-Man-(1-3)-(α-Man-(1-6)-)α-Man-(1-4)-β-GlcANAc3NAc-(1-4)-β-GlcNAc3NAc) |
GlyTouCan | G06484YZ G62526ZK |
Literature | |
Year of Identification | 2017 |
Year of Validation | 2017 |
Reference | Fujinami, D., Taguchi, Y. and Kohda, D., 2017. Asn-linked oligosaccharide chain of a crenarchaeon, Pyrobaculum calidifontis, is reminiscent of the eukaryotic high-mannose-type glycan. Glycobiology, 27(8), pp.701-712. |
Corresponding Author | Daisuke Kohda |
Contact | 1. Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. 2. Research Center for Advanced Immunology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. 3. Research Center for Live-Protein Dynamics, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. |