| Organism Information |
| Organism Name | Methanococcus voltae PS |
| Clinical Implication | Non-pathogenic |
| Domain | Archaebacteria |
| Phylum | Euryarchaeota |
| Classification | Family: Methanococcaceae
Order: Methanococcales
Class: Methanococci or Methanothermea
Division or phylum: "Euryarchaeota" |
| Taxonomic ID (NCBI) | 523842 |
| Genome Information |
| Gene Information |
| Gene Name | aglB |
| NCBI Reference Sequence | ABD17750 |
| Protein information |
| Protein Name | AglB |
| UniProtKB/ SwissProt ID | Q2EMT4 |
| UniProtKB Sequence | >sp|Q2EMT4|AGLB_METVO Dolichyl-phosphooligosaccharide-protein glycotransferase OS=Methanococcus voltae GN=aglB PE=1 SV=1
MTENNEKVKNSDSANNQSSKNSKFNFNFEDKKVKCAKTILIIIFLAFLSFQMRAQTADMG
FTTNEQYLDVFSDDNGRMYLTALDPYYYLRMSENYLENGHTGDTLKNIDGQQVPWDSYKY
GPTGARATFNLLSVVTVWVYQVWHAMDSTVTLMNAAFWVPAILSMFLITPIFFTVRRITS
SDIGGAVAAILASLSPSIFVKTVAGFSDTPILEILPLLFIVWFIIEAIHYSKEKNYKSLI
YGLLATLMLALYPFMWSAWWYGYYIVIAFLVIYAIYKGISYNSIAKYTKSKNNNHKDKIE
SEKLEMLNILKISGLFIIGGAVLITALYGVSTTMNALQAPLNYLGLDEVSSQTGWPNVLT
TVSELDTASLDEIISSSLGSIHLFAIGLIGIFLSLFRKVLTPVKQISNGLAEKLDIKYAL
LLIIWFAVTFLAASKGVRFVALMVPPLSIGVGIFVGFIEQFIKNNLDKKYEYVAYPTIAI
IVLYALFTIYRADSADLVRMLLPSNYVPIAEGIMLASLAVLIIYKVAELIAESNKKLVMN
KIFMILLAIGLITPTIATIVPFYSVPTYNDGWGESLEWINTQTPNNSVVTCWWDNGHIYT
WKTDRMVTFDGSSQNTPRAYWVGRAFSTSNESLANGIFRMLASSGDKAYTTDSVLIKKTG
SIKNTVDVLNEILPLTKSDAQKALKNSSYKFTDTEVSEILDATHPKVTNPDYLITYNRMT
SIASVWSYFGNWDFNLPAGTSRSEREAGSFQGLQTYATNINDTLIVRSLIQQTAEYNIYT
LIEVRNETLTGAMMAVTNDGQMQTQQLNMHKVKLMVNENGKSKMYNSLADPDGQLSLLIK
VDKNSIIGTDGSNNPVYSSSSWMATANLEDSVYSKLHFFDGEGLDTIKLEKESLDPTANG
VQPGFKVFSVDYGNYSK
|
| EMBL CDS | ABD17750.1 |
| Sequence length | 917 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) AglB is OSTase transfer the trisaccharide to the S-Layer protein and flagellin. |
| Additional Information | 1) M. voltae AglB has relaxed specificity and attaches incomplete glycans on flagellins and S-layer proteins.
2) AglB is a homolog of the eukaryotic (STT3p) and bacterial (PglB) oligosaccharyl transferases and contain conserved region WWDYG in its active site. |
| Glycosyltransferase Information |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser/Thr |
| Donor Type | Lipid linked sugars |
| Donor Specificity | DolP-GlcNAc-Glc-2,3-diNAcA |
| Accessory GT ID | ProGT16.1 ProGT16.2 ProGT16.3 ProGT16.4 |
| Glycan Information |
| Glycan transferred | Trisaccharide (ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc) |
| Method of Glycan Indentification | Nano-LC-MS/MS and NMR |
| Experimental_strategies | In vitro and In vivo |
| Acceptor Subtrate Information |
| Acceptor Substrate name | FlaA |
| ProGPdb ID | ProGP238 |
| Acceptor Substrate name | FlaB1 |
| ProGPdb ID | ProGP238 |
| Acceptor Substrate name | FlaB2 |
| ProGPdb ID | ProGP239 |
| Acceptor Substrate name | FlaB3 |
| ProGPdb ID | ProGP240 |
| Acceptor Substrate name | FlaA |
| ProGPdb ID | ProGP241 |
| Acceptor Substrate name | S-layer protein |
| ProGPdb ID | ProGP242 |
| Litrature |
| Year Of Validation | 2006 |
| Reference | Larkin, A., Chang, M. M., Whitworth, G. E., & Imperiali, B. (2013). Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis. Nature chemical biology, 9(6), 367.
|
| Authors | Larkin, A., Chang, M. M., Whitworth, G. E., & Imperiali, B.
|
| Research groups | Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
|
| Corresponding Author | Imperiali, B.
|
| Contacts | Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
|
| Reference | Chaban, B., Voisin, S., Kelly, J., Logan, S. M., & Jarrell, K. F. (2006). Identification of genes involved in the biosynthesis and attachment of Methanococcus voltae N?linked glycans: insight into N?linked glycosylation pathways in Archaea. Molecular microbiology, 61(1), 259-268.
|
| Authors | Chaban, B., Voisin, S., Kelly, J., Logan, S. M., & Jarrell, K. F.
|
| Research groups | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada
|
| Corresponding Author | Jarrell, K. F.
|
| Contacts | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada
|
| Reference | Shams-Eldin, H., Chaban, B., Niehus, S., Schwarz, R. T., & Jarrell, K. F. (2008). Identification of the archaeal alg7 gene homolog (encoding N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementation in Saccharomyces cerevisiae. Journal of bacteriology, 190(6), 2217-2220.
|
| Authors | Shams-Eldin, H., Chaban, B., Niehus, S., Schwarz, R. T., & Jarrell, K. F.
|
| Research groups | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada
|
| Corresponding Author | Jarrell, K. F.
|
| Contacts | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada
|
