| ProGT ID | ProGT45 (TpeL) |
| Organism Information |
| Organism Name | Clostridium perfringens |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Phylum: Firmicutes |
| Taxonomic ID (NCBI) | 1502 |
| Genome Information |
| Gene Information |
| Gene Name | tpeL |
| NCBI Reference Sequence | AB262081 |
| Protein information |
| Protein Name | TpeL |
| UniProtKB/ SwissProt ID | A2PYQ6 |
| UniProtKB Sequence | >tr|A2PYQ6|A2PYQ6_CLOPF TpeL OS=Clostridium perfringens GN=tpeL PE=4 SV=1
MGLMSKEQLIILAKNSSPKEGEYKKILELLDEYNLLNNSVEKNSIDLYLKLNELSKSIDI
YLKKYKNSKRNNALYQLKSDLTKEVIEIKDTNLKPLEKNIHFVWVGGMINNISIDYINQW
KDINSDYETIIWYDSEALLVNILKKAIIDSSNKEVLTKYESVLNDNSFDSNKFYRERMEV
IFRKQKEFNNYYNTNDNYTKSLNDVIKVYLIEKYLKTDEELEKYINESKEVFKANGAKDI
REYDILDDVELKSIYEQELLMRFNLASASDIIRVIVLNKLGGIYLDVDVLPGIKKHIFKD
INKPTNISENKWQMIQLETIMKYKQYIKGYTENSFKNLPSDLQEMLQEKVVEKNLKSDIF
QRLGDIFISELDTKIAFMFGKIANQVLISKKNSYSLNLIINQIKNRYNIINKCLSSAIEK
GSNFNNTVDIFIQQLNEFYVNEGFFVSKVMGYLGDGYMPDMRATLNISGPGIYTAAYYDL
LYFNERSLNPQILQEDLKYFEVPQALISQQTEQEINSSWTFNQVKSQIEYKKLVEKYTNK
SLSENDKLNFNENKIIDKVELLNRINSNNLINFDDKEYLRYIIQLQGDKVSYEAAINLFI
KNPSNSILVQEINNISYYFNSEYKSIDSIQFDNIPEILKGKNKIKLTFIGHGEEEFNTER
FASLTVKEFSKKIYKVLDMIKSNTNVKEIQIDLLGCNMFSYNINVEETYPGKLLKVVLDY
VDKIYNADIKPEIKISANQYEVRINKDGKKELLSHSGEWLSKEEAIIKDIASKEIIYFDT
RENTIKAEAKNIMELITFRNSLDKKLNDLESLINNNFIVNEVYEQLTSNNLYRTSFRNSF
LDTINFIEDVSQELYEIKIKNNINDDYIMALDEIKQDNNISEIKFININSGEFRVVRENS
RLINKFKEDFKFMINNIKRLIKFSDNNKLITSFELKNIESSSGVSTLNTSFLIQSMIDYK
AQNFDFNKLSTSVKVQIYCQITNISLSEIQDASNLVKIIAEANEIEINLIPTLANAIPLI
TTIVDGINLIANIDELINTKDELLRKELAARIGIISSNMTAAISSYILYFTEFGEVFNPL
LVPIAGISSGIPTLVNNILILEEKSKEITEYFSHISKVESDGLFKVSDNNSILIPLDDIA
ISKIDFNKREVILDKLDIWAMEGGSGLSGKETFFSAPYINENLPKLSIDNLLKIDINKID
FSIKGMMLPNGVSKTLGYEFNTVANIYELENDGVNLLNRIRDNYPGQFYWRYYATLFNYG
MVNLKINYHDTDVKINLDNVDRMFIVPTLTIDQAREKLSYNFNGAGANYYIYLSSQPIKI
FINGTKEDNWILNIDDIVKEVTIVNNSIVRGKFIENIFKKLTIEDNKIIIGNQKIYLKNN
KTNIHFSVSILDGVNLMVEVDFNNKMYHLSLQGNEKTICNNMNIIQNNINKLFGSSNIKS
IPYFYKNNGLENCIGVFSIKNNEFIYMLKYDDKNKIYKYKDNNLVCGFNLFDISYVDIIS
NGDKNYLKGIWNTSVNNSEYKIEFLVELIDQNTIEIIKLNLSDGLIKSFLTILDNLESLD
VKISTIYDFLKNILFRDVFLENESLNFIVSKGFVLTGNNSKNKYEFICNSENIQLYITEL
YTNGTKFRNWLDNGKILVSSTGESSNNLIDI
|
| EMBL CDS | BAF46125.1 |
| Sequence length | 1651 AA |
| Subcellular Location | Host cell plasma membrane |
| Function in Native Organism | 1) It is a secretory glycosyltransferase which glycosylates the Rho family protein of host. |
| Additional Information | 1) TpeL is a glycosyltransferase uses UDP-GlcNAc and UDP-Glc as donor co-substrates and Rac and Ras subfamily protein as acceptor substrates. |
| Glycosyltransferase Information |
| Glycosylation Type | O- (Thr) linked |
| CAZY Family | GT44 |
| EC Number (BRENDA) | 2.4.99.18.11923 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-Glc and UDP-GlcNAc |
| Glycan Information |
| Glycan transferred | Monosaccharides (Glc and GlcNAc) |
| Method of Glycan Indentification | MS and MS/MS and MALDI TOF/TOF |
| Experimental_strategies | In vitro |
| Acceptor Subtrate Information |
| Acceptor Substrate name | Ras (cH-Ras, N-Ras, and K-Ras) |
| Acceptor Substrate name | RalA |
| Acceptor Substrate name | Rap1B |
| Acceptor Substrate name | Rac1 |
| Litrature |
| Year Of Validation | 2010 |
| Reference | Nagahama, M., Ohkubo, A., Oda, M., Kobayashi, K., Amimoto, K., Miyamoto, K., & Sakurai, J. (2011). Clostridium perfringens TpeL glycosylates the Rac and Ras subfamily proteins. Infection and immunity, 79(2), 905-910.
|
| Authors | Nagahama, M., Ohkubo, A., Oda, M., Kobayashi, K., Amimoto, K., Miyamoto, K., & Sakurai, J.
|
| Research groups | Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan
|
| Corresponding Author | Sakurai, J.
|
| Contacts | Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan
|
| Reference | Guttenberg, G., Hornei, S., Jank, T., Schwan, C., Lü, W., Einsle, O., Papatheodorou, P. & Aktories, K. (2012). Molecular characteristics of Clostridium perfringens TpeL toxin and consequences of mono-O-GlcNAcylation of Ras in living cells. Journal of Biological Chemistry, jbc-M112.
|
| Authors | Guttenberg, G., Hornei, S., Jank, T., Schwan, C., Lü, W., Einsle, O., Papatheodorou, P. & Aktories, K.
|
| Research groups | Institute of Experimental and Clinical Pharmacology and Toxicology
|
| Corresponding Author | Aktories, K.
|
| Contacts | Institute of Experimental and Clinical Pharmacology and Toxicology
|
| Reference | Pauillac, S., Dallayer, J., Lenormand, P., Rousselle, J. C., Bouvet, P., & Popoff, M. R. (2013). Characterization of the enzymatic activity of Clostridium perfringens TpeL. Toxicon, 75, 136-143.
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| Authors | Pauillac, S., Dallayer, J., Lenormand, P., Rousselle, J. C., Bouvet, P., & Popoff, M. R.
|
| Research groups |
Institut Pasteur, Anaerobic Bacteria and Toxins Unit, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
|
| Corresponding Author | Popoff, M. R.
|
| Contacts | Institut Pasteur, Anaerobic Bacteria and Toxins Unit, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
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