| ProGT ID | ProGT64 (TcsH) |
| Organism Information |
| Organism Name | Paeniclostridium sordellii strain VPI 9048 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Phylum: Firmicutes |
| Taxonomic ID (NCBI) | 1505 |
| Genome Information |
| Gene Bank | KC814176.1 |
| EMBL | KC814176 |
| Gene Information |
| Gene Name | tcsH |
| NCBI Reference Sequence | KC814176 |
| Protein information |
| Protein Name | TcsH |
| UniProtKB/ SwissProt ID | M9ZTT7 |
| UniProtKB Sequence | >tr|M9ZTT7|M9ZTT7_PAESO Haemorrhagic Toxin OS=Paeniclostridium sordellii GN=tcsH PE=4 SV=1
MSLISKDELIKLAYSIKPREDEYKTILTNLDEYNKLVTINNKDKYLQLKKLNDSIDIFIN
KYKKSSRNRALFNLKKDISKEVILIKNSNISPVEKNLHFVWIGGEVSDTALEYINQWADI
NRDYNIRVWYDGEAFLVNTLKKAIVEHSTTDTLQLFEEDIKNPQFDNMKFYKKRMEFIYE
RQNRFINYYKSEINKPIKPTIDDIIKSHLVSEYNKNSESLELYRRTSFEKISNNNGVDIR
NNNLFTEQELLNIYNQELLDRENLAAASDLVRLLALKDFGGVYLDVDMLPGIQPDLFKTI
SRPSSIGVDSWEMIKLEAIMKYKKYIKNYTSKNFDKLDQQLKDDFQITLESKSEKSEIFS
KLGNLDVSDLEIKIAFALGSVINQALISKKGSYLTNLVIEQVKNRYKFLNQHLNPAIELG
GNFSDTTKNFHDSLFNSATSENSMFLTKIASYLQVGFMPEARATISLSGPGAYSSAYYDF
INLQDNTIEKTLNASDLMEFKFPESNLSQFTEQEINSLWSFDQASAKYQFEKYVRDYTQE
SLSEDSELDFNKNTVLDKNYLLNNKIPSNNVEETGSKNYVHYIIQLQGDDISYESACNLF
SKNPKNSVIIQRNMNESAKSYFLSDNGESISELNKYRIPQRLKNKEKIKVTFIGHGKDEF
NTSEFAKLSVDSLSNEISSFLDIMKLDISPKNVEINLLGCNMFSYNVNVEETYPGKLLLN
NIDKIISTLPNVNKDNITIGANQYEVRINNEGRKELLDHSGQWLNKEEAIMNDLSSKEYI
FFDSIENRPKAKSKNLIELASISDNIKTLLLDTNIDPETKFILNNLKLNIESSIDNNIYY
EKLEPVKNIIHNSIDNLTNEFNLIENVSDELYELKKINNLDDNYLISFEDISKNDSTYTI
RFINKNSGESVHIQTEKEIFLKYGEHITQEINTIKNNIIIDVNGNLIGNIELEHAPQVNT
LNAAFFIQSLIDYSNNKDVLNNLRTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAINDA
INVLPTLTEGVPILATILDGISLGAAIKELSETNDPLLKKELEAKVGIIAINMSLSLASM
ISTVIGVGSELAIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFTHLSESKKYGPFKL
EDDKILSPIDDLVISEIDFNTDSIKLGTCDILSMEGGSGYTVTNDIDHFFSSPYINSNIP
PLSIYPVMNIQTTNLDFSKDLMMLPNAPSRLLWWETGAVPGLRSLETDGTRLLDSIRDFY
PGKFYWRFYAWFDFAITTLKPVYENTNIKIKLDKNTRNFIMPTITTDVIRNNLSYSFTGS
GGTYSLLLSSHPISTNINLSKDDLWIFNIDNKVREISIQNGTIKKGNLVRNALSNLDINK
NKLTIDNQIINFSGDVDNKYRYIFLNCSLDDEISLMIEINLFAKSYNLILSGNKDYLISN
LSTIINKINNLGLNSKNISYNYTDEFNNKYFGVISKTNQKSIICYKKGSKNILELYNGNM
LLFDSKDFIADDINIFMKDDINTITGKYYIDNNLDISVDFSISLISKNKVKVNGLYLNEY
GYASFLEFIKNSDGHHNTSNFINLFLDNIGFWKLFGFNNIEFVIDKYFAITGKTDMGYIE
FICDNNKNIDIYFGEWKTSSTKNTIFSGNGRNLIVEPIYDINAGDNISTSIDFSYEYISG
IDIYINKILIVPNLYTELVNINTDYSSNKYFPEIIVLNTDTFHDKVNINLDSSSLDYEWA
IDGSDFILSRYLEENNRKILQKIRIKDILSNTKSFNKMMIDFKDIKNISLDHIMNNFKSF
NSESELDRDHFGFKTIDSKTYYYNEVGKLVKGLININDSLFYFDTIESNLVTGWKTINGK
TYYFDINNGVAYIGYKTIDGKNFYFDGNGIMQIGVFKVPDGFKYFAPANTYNNNEEGQTI
VYQNKFLTINGKKYYFDNNSKAVTGWQIINGNKYYFDTNTGIAAVGLQIINNNKYYFNPH
TAIAATGWQSINNAKYYFDINTYIGTTGYKTIDSKNFYFDSNCIMQIGVFKVSDGFKYFA
PANTYNNNEEGQAIVYQNKFLTINGKRYYFDNNSKAVTGWHTIDGKKYYFNPNTAIAATG
WHTIDDKKYYFNPDTAIAATGWHTIDDKKYYFNPNTGITSTGETTINNKSFYFNDKGIMQ
IGVFKVPDGFKYFAPANTHNNNLEGQAIVYQNKFLTINGKKYYFDNNSKAITGWQVIDDK
KYYFNSNTAVADTNLCTINNEKYYFSYDGILQNGYITIGRLNFYFDSNNDSKMTTGVFKG
PNGFEYFAPANTYNNNLEGQAIVYQNKFLTINGKKYYFDNKSKAVTGWQTIDGKKYYFNP
NTAIAAMGWQAIDGKKYYFNPNTAIATTGWQTIDGKKYYFNPNTAIAATGWQAIDGKKYY
FNPNTATTSIGYTTINSKNFYFNNDGIMQLGVFKGPDGFEYFAPANTHNNNEEGQSITYQ
NKFLIFNEDVYYFDSSSKAVTGWRTIDDHRFYFEPNTGIGANGYKTLDGKNFYFRNGLPQ
FGVFKGPDGFEYFAPANTHNNNEEGQSITYQNKFLVFLGNRYYFDSSSKAVTGWQTINGN
TYYFMPDTAIAAAGGFFTIDGAIYFFGIDGVKQPGIYG
|
| EMBL CDS | AGK40891 |
| Sequence length | 2618 AA |
| Subcellular Location | Host cell plasma membrane |
| Function in Native Organism | 1) It is a secretory glycosyltransferase which glycosylates the Rho family protein of host. |
| Potential Application | 1) Potential veterinary vaccine candidate against Clostridium sordellii infections. |
| Additional Information | 1) According to sequence similarity, TcsH is highly related to Toxin B (TcdB) from C. difficile
2) In vitro studies show that it glycosylate Rho-GTPases and Ras-GTPases of host protein during infection.
3) Recombinant LCGT transferase domains (rN) rN-TcsH-9048 glucosylated preferably Rho-GTPases but also Ras-GTPases to some extent.
4) rN-TcsH-9048 differ from the respective full-length TcsH-9048, which exclusively glucosylate Rho-GTPases.
5) Vero cells treated with full-length TcsH-9048 showed glucosylation of Ras, albeit to a lower extent than of Rho-GTPases. |
| Glycosyltransferase Information |
| Glycosylation Type | O- (Thr) linked |
| CAZY Family | GT44 |
| EC Number (BRENDA) | 2.4.1.B62.13625. |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-Glc and UDP-GlcNAc |
| Glycan Information |
| Glycan transferred | Monosaccharides (GlcNAc and Glc) |
| Experimental_strategies | In vitro |
| Acceptor Subtrate Information |
| Acceptor Substrate name | RhoA |
| Acceptor Substrate name | Rac1 |
| Acceptor Substrate name | Ras |
| Acceptor Substrate name | Cdc42Hs |
| Litrature |
| Year Of Validation | 2013 |
| Reference | Thiele, T. L., Stuber, T. P., & Hauer, P. J. (2013). Detection of Clostridium sordellii strains expressing hemorrhagic toxin (TcsH) and implications for diagnostics and regulation of veterinary vaccines. Vaccine, 31(44), 5082-5087.
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| Authors | Thiele, T. L., Stuber, T. P., & Hauer, P. J.
|
| Research groups | Center for Veterinary Biologics, Animal and Plant Health Inspection Service, United States Department of Agriculture, 1920 Dayton Avenue, Ames, IA 50010, USA
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| Corresponding Author | Hauer, P. J.
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| Contacts | Center for Veterinary Biologics, Animal and Plant Health Inspection Service, United States Department of Agriculture, 1920 Dayton Avenue, Ames, IA 50010, USA
|
| Reference | Genth, H., Pauillac, S., Schelle, I., Bouvet, P., Bouchier, C., Varela?Chavez, C., Just, I. & Popoff, M. R. (2014). Haemorrhagic toxin and lethal toxin from C lostridium sordellii strain vpi9048: molecular characterization and comparative analysis of substrate specificity of the large clostridial glucosylating toxins. Cellular microbiology, 16(11), 1706-1721.
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| Authors | Genth, H., Pauillac, S., Schelle, I., Bouvet, P., Bouchier, C., Varela?Chavez, C., Just, I. & Popoff, M. R.
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| Research groups | Institute of Toxicology, Medical School Hannover, Hannover, Germany.
|
| Corresponding Author | Popoff, M. R.
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| Contacts | Institute of Toxicology, Medical School Hannover, Hannover, Germany.
|
| Reference | Ziegler, M. O., Jank, T., Aktories, K., & Schulz, G. E. (2008). Conformational changes and reaction of clostridial glycosylating toxins. Journal of molecular biology, 377(5), 1346-1356.
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| Authors | Ziegler, M. O., Jank, T., Aktories, K., & Schulz, G. E.
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| Research groups | Institute of Organic Chemistry and Biochemistry, Albert-Ludwigs-University, Albertstr. 21, D-79104 Freiburg im Breisgau, Germany.
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| Corresponding Author | Schulz, G. E.
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| Contacts | Institute of Organic Chemistry and Biochemistry, Albert-Ludwigs-University, Albertstr. 21, D-79104 Freiburg im Breisgau, Germany.
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| Reference | Huelsenbeck, S. C., Klose, I., Reichenbach, M., Huelsenbeck, J., & Genth, H. (2009). Distinct kinetics of (H/K/N) Ras glucosylation and Rac1 glucosylation catalysed by Clostridium sordellii lethal toxin. FEBS letters, 583(19), 3133-3139.
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| Authors | Huelsenbeck, S. C., Klose, I., Reichenbach, M., Huelsenbeck, J., & Genth, H.
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| Research groups | Institute of Toxicology, Hannover Medical School, D-30625 Hannover, Germany
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| Corresponding Author | Genth, H.
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| Contacts | Institute of Toxicology, Hannover Medical School, D-30625 Hannover, Germany
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