ProGT72 (Tot/AglB)
| ProGT ID | ProGT72 (Tot/AglB) |
| Organism Information | |
| Organism Name | Ralstonia solanacearum GMI1000 |
| Clinical Implication | Non-pathogenic |
| Domain | Archaebacteria |
| Phylum | Euryarchaeota |
| Classification | Family: Halobacteriaceae Order: Halobacteriales Class: Halobacteria Phylum: Euryarchaeota |
| Taxonomic ID (NCBI) | 272569 |
| Genome Information | |
| Gene Bank | AY596297 |
| EMBL | AY596297 |
| Gene Information | |
| Gene Name | tot |
| NCBI Gene ID | 3130386 |
| NCBI Reference Sequence | AY596297 |
| Protein information | |
| Protein Name | Tot/AglB |
| UniProtKB/ SwissProt ID | Q5V4T7 |
| NCBI Ref Seq | WP_011223023.1 |
| EMBL CDS | AAV45465.1 |
| Sequence length | 937 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| String | 272569.rrnAC0431 |
| Additional Information | 1) AglB of Har. marismortui is able to complement Hfx. volcanii AglB in AglB deleted Hfx. Volcanii cell. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser/Thr |
| Donor Type | Lipid linked sugars |
| Glycan Information | |
| Glycan transferred | Pentasaccharide comprising a Monosaccharide ( hexose), two hexuronic acids, a methyl ester of hexuronic acid, and a Monosaccharide (Man) |
| Method of Glycan Indentification | LC-ESI MS/MS |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | S-layer glycoprotein |
| ProGPdb ID | ProGP71 |
| Litrature | |
| Year Of Validation | 2014 |
| Reference | Cohen-Rosenzweig, C., Guan, Z., Shaanan, B., & Eichler, J. (2013). Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans. Applied and environmental microbiology, AEM-03191. |
| Authors | Cohen-Rosenzweig, C., Guan, Z., Shaanan, B., & Eichler, J. |
| Research groups | Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel. |
| Corresponding Author | Eichler, J. |
| Contacts | Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel. |