ProGP142 (Antifreeze protein)
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| ProGP ID | ProGP142 (Antifreeze protein) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Pseudomonas putida GR12-2 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Gammaproteobacteria Orders : Pseudomonadales Family : Pseudomonadaceae Genus : Pseudomonas Species : putida Strain : GR12-2 |
| Taxonomic ID (NCBI) | 303 |
| Genome Information | |
| GenBank | AJ784158 |
| EMBL | AJ784158 |
| Gene Information | |
| Gene Name | afpA |
| Protein Information | |
| Protein Name | Antifreeze protein |
| UniProtKB/SwissProt ID | Q68VA9 |
| EMBL-CDS | CAH04619.1 |
| UniProtKB Sequence | >tr|Q68VA9|Q68VA9_PSEPU Antifreeze protein OS=Pseudomonas putida GN=afpA PE=4 SV=1 MQYDSPITNTEFQTFLTTSSISDDTAAAISTLLNLDSADTINLASWDGVNAPEIPTGQEG AADVVIVNVPGAATDLVPVEIPDSLNSAKAFIFDSNASLAVTFDAPVAAESASLARVAAD TTAGIEFLVTTGAGNDVITVNGDQNSYIDAGNGNDTIVTGNGNNTVVAGAGNNNVTTGTG NDTIILSGTGHTDIVNTGAGYDVVQLDGSAADYTITAGNSNNVTLTGAQTAAITGAEFLT FADGSSVALAQSEAEASALRLYEGILGRDADQGGAQNFIAQVEAGTALTDIANSFLNSAE FGGAATEASIDSLYTSLLGRGADTAGSDSWEAIIANGGSLADVAAGIAGSAEAQEQDQSN GTFVDSLYLNALGRPSDEAGHDAWVAQLFNGASRAEVAAGIVGSAEAAEKINSDFIDALY LSATGRASDEAGKAGWTEVLANGGTQADVAIGIVGSQEAIAHNDNVVVLHGAV |
| Sequence length | 473 AA |
| Subcellular Location | Secreted |
| Function | This 164-kDa glycoprotein has both antifreez activity and ice-nucleation activity. |
| Glycosylation Status | |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining |
| Glycan Information | |
| Glycan Annotation | Glycosylation does not seem to have any effect on antifreez activity while its removal decreases the ice-nucleation activity. The protein is relatively rich Gly and Ala. |
| Literature | |
| Year of Identification | 1998 |
| Year of Identification Month Wise | 1998.01 |
| Reference | Xu, H., Griffith, M., Patten, C.L. and Glick, B.R., 1998. Isolation and characterization of an antifreeze protein with ice nucleation activity from the plant growth promoting rhizobacterium Pseudomonas putida GR12-2. Canadian Journal of Microbiology, 44(1), pp.64-73. |
| Corresponding Author | Bernard R Glick |
| Contact | Department of Biology, University of Waterloo, Waterloo,ON N2L 3G1, Canada |