ProGP31 (Flagellin B2)

Home -> ProGPdb -> Search ProGP -> Display data


ProGP ID ProGP31 (Flagellin B2)
Validation Status Characterized
Organism Information
Organism NameHalobacterium salinarum (Halobium) R1M1/NRC-1
Domain Archaea
Classification Phylum : Euryarchaeota
Class : Halobacteria
Orders : Halobacteriales
Family : Halobacteriaceae
Genus : Halobacterium
Species : salinarum
Strain : R1M1/NRC-1
Taxonomic ID (NCBI) 64091
Genome Information
GenBank AE004437.1
EMBL AE004437
Gene Information
Gene NameflaB2 (VNG_0961G)
NCBI Gene ID 1447686
GenBank Gene Sequence NC_002607.1
Protein Information
Protein NameFlagellin B2
UniProtKB/SwissProt ID P13077
NCBI RefSeq WP_010902681.1
EMBL-CDSAAG19384.1
UniProtKB Sequence >sp|P13077|FLAB2_HALSA Flagellin B2 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB2 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVDTSGSTEVVNYANLTVRQAAGADNINLSKSTIQWIGPDTATTLTYDGTT ADAENFTTNSIKGDNADVLVDQSDRIEIVMDAAEITTNGLKAGEEVQLTVTTQYGSKTTY WANVPESLKDKNAVTL
Sequence length 196 AA
Subcellular LocationFlagellum
Function Flagellin is the structural subunit of the flagellar filaments.
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN97, N125
Experimentally Validated Glycosite(s ) in Mature ProteinN97, N125
Glycosite(s) Annotated Protein Sequence >sp|P13077|FLAB2_HALSA Flagellin B2 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB2 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVDTSGSTEVVNYANLTVRQAAGADNIN*(97)LSKSTIQWIGPDTATTLTYDGTT ADAEN*(125)FTTNSIKGDNADVLVDQSDRIEIVMDAAEITTNGLKAGEEVQLTVTTQYGSKTTY WANVPESLKDKNAVTL
Sequence Around Glycosites (21 AA) VRQAAGADNINLSKSTIQWIG
TYDGTTADAENFTTNSIKGDN
ProGP Web Logo
Technique(s) used for Glycosylation DetectionMass shift on SDS-PAGE after deglycosylation with anhydrous hydrogen fluoride. LC ESI MS, MS/MS
Technique(s) used for Glycosylated Residue(s) Detection Glycopeptide sequencing
Glycan Information
Glycan Annotation Linkage: Glc-Asn.
Sulfated oligosaccharides that resemble those of the cell-suface glycoprotein in the bacterium are present. Glucose and glucuronic acid are the constituents of the glycans which are of the type GlcA-(1→4)-GlcA-(1→4)-GlcA-(1→4)-Glc.
BCSDB ID 136320
GlyTouCan G81788YA
Technique(s) used for Glycan Identification GLC-MS (gas liquid chromatography-mass spectrometry) after perfluoropropionylation.
Protein Glycosylation linked (PGL) gene(s)
Additional CommentRemoval of Mg++ ions in the growth medium inhibits glycosylation of proteins in vivo. This leads to the reduction in the molecular masses of newly synthesized flagellins. There might be another modification of the flagellins that is resistant to HF treatment. Halobacterial glycoproteins including flagellins are glycosylated at the extracellular surface of the cell membrane.
Literature
Year of Identification1985
Year of Identification Month Wise1985.12
Year of Validation 1989
ReferenceCohen-Krausz, S. and Trachtenberg, S., 2002. The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. Journal of molecular biology, 321(3), pp.383-395.
Corresponding Author Shlomo Trachtenberg
ContactDepartment of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel.
ReferenceGerl, L., Deutzmann, R. and Sumper, M., 1989. Halobacterial flagellins are encoded by a multigene family Identification of all five gene products. FEBS letters, 244(1), pp.137-140.
Corresponding Author Manfred Sumper
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany.
ReferenceGerl, L. and Sumper, M., 1988. Halobacterial flagellins are encoded by a multigene family. Characterization of five flagellin genes. Journal of Biological Chemistry, 263(26), pp.13246-13251.
Corresponding Author Manfred Sumper
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany.
ReferenceWieland, F., Paul, G. and Sumper, M., 1985. Halobacterial flagellins are sulfated glycoproteins. Journal of Biological Chemistry, 260(28), pp.15180-15185.
Corresponding Author Felix Wieland
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany
ReferenceVershinin, Z., Zaretsky, M., Guan, Z. and Eichler, J., 2021. Revisiting N-glycosylation in Halobacterium salinarum: Characterizing a dolichol phosphate-and glycoprotein-bound tetrasaccharide. Glycobiology.
Corresponding Author Jerry Eichler
ContactDepartment of Life Sciences, Ben Gurion University of the Negev, PO Box 653, Beersheva 84105, Israel.