ProGP104 (S-layer glycoprotein (138 kDa))

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ProGP ID ProGP104 (S-layer glycoprotein (138 kDa))
Validation Status Characterized
Organism Information
Organism NameMethanoculleus (Methanogenium) marisnigri JR1
Domain Archaea
Classification Phylum : Euryarchaeota
Class : Methanomicrobia
Orders : Methanomicrobiales
Family : Methanomicrobiaceae
Genus : Methanoculleus
Species : bourgensis
Strain : JR1
Taxonomic ID (NCBI) 1201294
Genome Information
GenBank NC_018227.2.
EMBL HE964772
Gene Information
Gene NameMEMAR_RS02690
NCBI Gene ID 76731121
GenBank Gene Sequence NC_009051.1
Protein Information
Protein NameS-layer glycoprotein (138 kDa)
NCBI RefSeq WP_011843393.1
Sequence length 773 AA
Subcellular LocationSurface
Function Component of the hexagonally arranged surface layer. High proportion of acidic amino acids and little, or no, sulfur-containing amino acids.
Glycosylation Status
Glycosylation Type O- (Thr) linked & N- (Asn) liked
Experimentally Validated Glycosite(s) in Full Length ProteinN78, N294, N383, N412, N568, N325, T326, S327, N188, G189, T190, N307, T386
Technique(s) used for Glycosylation DetectionAlcian Blue 8GX and thymol sulfuric acid staining.
Glycan Information
Glycan Annotation 0.8% galactose, 0.3% glucose, and 0.1% mannose.
Technique(s) used for Glycan Identification NanoLC-MS/MS analysis, NMR analysis
Year of Identification1994
Year of Identification Month Wise1994.03
ReferenceBayley, D.P. and Koval, S.F., 1994. Membrane association and isolation of the S-layer protein of Methanoculleus marisnigri. Canadian journal of microbiology, 40(3), pp.237-241.
ReferenceKelly, J., Vinogradov, E., Robotham, A., Tessier, L., Logan, S.M. and Jarrell, K.F., 2022. Characterizing the N-and O-linked glycans of the PGF-CTERM sorting domain-containing S-layer protein of Methanoculleus marisnigri. Glycobiology.
Corresponding Author John Kelly
ContactHuman Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada