ProGP108 (Tetrabrachion (S layer glycoprotein))

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ProGP ID ProGP108 (Tetrabrachion (S layer glycoprotein))
Validation Status Characterized
Organism Information
Organism NameStaphylothermus marinus F1
Domain Archaea
Classification Phylum : Crenarchaeota
Class : Thermoprotei
Orders : Desulfurococcales
Family : Desulfurococcaceae
Genus : Staphylothermus
Species : marinus
Strain : F-1
Taxonomic ID (NCBI) 2280
Genome Information
GenBank U57967
EMBL U57967
Gene Information
Gene NameSmar_1008
Protein Information
Protein NameTetrabrachion (S-layer glycoprotein)
UniProtKB/SwissProt ID Q54436
EMBL-CDSAAC44118.1
UniProtKB Sequence >tr|Q54436|Q54436_STAMA Tetrabrachion OS=Staphylothermus marinus PE=1 SV=1 MNRVLAYSLLAIMTLSLLIIPAPGIAQRITVGVSVKAGTYNFYNITPTTQTVEVTDNGML RVIINRTEATELGTTIRLAFILDTDKYDPNVGGYFLNVSNIGVYAPSDPTQSPYGGVIDI TQNSTLTDGTQVVGNVTVVNGGNNVIILIDLSKLPDLQNVVYITNVYTETSTTANLTNTL LRVKAFDAASWDAVISGNQFKILYIPSLCKYVKINVIHSPAIVGTNVDVIVSFHKYFSLV QSIAGVSLDITVDNKTQLNMTNYYNATENYVLATFINGNLTVGGSEVFSSPTVTVINAST FKYSGQVKDYAPTVADTATPWVRTLNKFEVEFRHEIVNXTHDLIFYIHCDSDTVSYDTWP FLIVNASLDITTTEVAFNSTTINPGDIVNFTAHNVPLQYLTATNYGVLRFQLINPALVVY VPVSNMTLSANTTTGIINGSFVLPDAPYGGLDYLTYLVFNDGKFIANGYITVSPCIETYV LTNTSAYAEDAGSSYIGRFVPGYTSVPGDYIVIKGYGFALSNLTGFTVSINNTDVIILNA TYNASTGKIIILAKLLDTNGTPIPVGAGFIRVGQNGTTNIAYAPFNVTRNSGLEKVLFNP RWFYNGTYYIEHDKLGDPYLYFPVDYPLVNNTFTTEMWPFNTTIEVIGWPTNTFTLKAFN KEFNLSFNLLTLSLTNGYNMTNLYNLTIPFLPYGNYTLLEGTLLSVNNRTVFTVHMGINV DLDSCGNGTLSITVVGAAPNTEYNFTFGYQVHDLNYGITRYISPQWNGTWNISLVTDIYG TGSTSVPLITLYPTSYVINATWDVITWLRLSGSGTLDLLFSVDVSYNGFTDNLTTPITYV FGPSDTTPGSFNIYVNTTYNVSVVRVAVDYLPRTNVVISVPETVLPGDTITVQIFPHHNE VWGFIEPTALFDENQLLGWYLTVRLVDPLSNTVVERVAGYYAGNLIVEDVDGDGDNEVWF VVNLTAPLVLGVDKTYRVDVELFLAVLNPSSNITGVTAVDNECYVQLDLNGTIYWNGLGS GIMLGGDGQIVTVLGVLEGKLDTIKDGIAEINATVNDINTYLKVNVTDLLKTINNSVVMI KNDTATLIIGQAEIKAKLDDLLNLTSQVNDTVTMILACCNNASKVLNRMEGTLNSTYTGV LNVKSDLSTLIDTANNVVIPKFNELYDNVTVEINASRDLIIQKISSVNDSLTTIISAGFN DVEAMISNLNTTLLNRIDELEGTLLFYMTANEQRLEGIINETADDIVYRLTVIIDDRYES LKNLITLRADRLEMIINDNVSTILASIGNVNLTVFNKLNDLEIELGDVNATINAGIFQIQ SNLGNANQLILDTLTSSKVEILNAISSNASAISSEIHNAVNQLSTLVLQVNDTLTLKITG EADNILNFLSSLEGSMNTGFNNVTSTLSAVENNILGKITDTSNLLSSKIDNTLSTLQDLI TSTSNDLKNSISSAKNDIVSSLSSKVDSSTQTLSTKLDDLKSAQESNTNSINNNIMLFGA ASLILLIVTIGLVGYRLIARRRVG
Sequence length 1524 AA
Subcellular LocationSurface (anchored in the membrane at one end)
Function Structural unit of S layer making it stable towards extremely harsh conditions. Encoded as a precursor with 26 AA signal peptide.
Protein Structure
PDB ID 1FE6, 1YBK
Glycosylation Status
Glycosylation Type N- (Asn) linked, (O- (Ser/Thr) linked residues not yet identifi
Experimentally Validated Glycosite(s) in Full Length Protein N44, N605, N641, N685, N708, N1279, N1402
Experimentally Validated Glycosite(s ) in Mature Protein N44, N605, N641, N685, N708, N1279, N1402
Glycosite(s) Annotated Protein Sequence >tr|Q54436|Q54436_STAMA Tetrabrachion OS=Staphylothermus marinus PE=1 SV=1 MNRVLAYSLLAIMTLSLLIIPAPGIAQRITVGVSVKAGTYNFYN*(44)ITPTTQTVEVTDNGML RVIINRTEATELGTTIRLAFILDTDKYDPNVGGYFLNVSNIGVYAPSDPTQSPYGGVIDI TQNSTLTDGTQVVGNVTVVNGGNNVIILIDLSKLPDLQNVVYITNVYTETSTTANLTNTL LRVKAFDAASWDAVISGNQFKILYIPSLCKYVKINVIHSPAIVGTNVDVIVSFHKYFSLV QSIAGVSLDITVDNKTQLNMTNYYNATENYVLATFINGNLTVGGSEVFSSPTVTVINAST FKYSGQVKDYAPTVADTATPWVRTLNKFEVEFRHEIVNXTHDLIFYIHCDSDTVSYDTWP FLIVNASLDITTTEVAFNSTTINPGDIVNFTAHNVPLQYLTATNYGVLRFQLINPALVVY VPVSNMTLSANTTTGIINGSFVLPDAPYGGLDYLTYLVFNDGKFIANGYITVSPCIETYV LTNTSAYAEDAGSSYIGRFVPGYTSVPGDYIVIKGYGFALSNLTGFTVSINNTDVIILNA TYNASTGKIIILAKLLDTNGTPIPVGAGFIRVGQNGTTNIAYAPFNVTRNSGLEKVLFNP RWFYN*(605)GTYYIEHDKLGDPYLYFPVDYPLVNNTFTTEMWPFN*(641)TTIEVIGWPTNTFTLKAFN KEFNLSFNLLTLSLTNGYNMTNLYN*(685)LTIPFLPYGNYTLLEGTLLSVNN*(708)RTVFTVHMGINV DLDSCGNGTLSITVVGAAPNTEYNFTFGYQVHDLNYGITRYISPQWNGTWNISLVTDIYG TGSTSVPLITLYPTSYVINATWDVITWLRLSGSGTLDLLFSVDVSYNGFTDNLTTPITYV FGPSDTTPGSFNIYVNTTYNVSVVRVAVDYLPRTNVVISVPETVLPGDTITVQIFPHHNE VWGFIEPTALFDENQLLGWYLTVRLVDPLSNTVVERVAGYYAGNLIVEDVDGDGDNEVWF VVNLTAPLVLGVDKTYRVDVELFLAVLNPSSNITGVTAVDNECYVQLDLNGTIYWNGLGS GIMLGGDGQIVTVLGVLEGKLDTIKDGIAEINATVNDINTYLKVNVTDLLKTINNSVVMI KNDTATLIIGQAEIKAKLDDLLNLTSQVNDTVTMILACCNNASKVLNRMEGTLNSTYTGV LNVKSDLSTLIDTANNVVIPKFNELYDNVTVEINASRDLIIQKISSVNDSLTTIISAGFN DVEAMISNLNTTLLNRIDELEGTLLFYMTANEQRLEGIINETADDIVYRLTVIIDDRYES LKNLITLRADRLEMIINDN*(1279)VSTILASIGNVNLTVFNKLNDLEIELGDVNATINAGIFQIQ SNLGNANQLILDTLTSSKVEILNAISSNASAISSEIHNAVNQLSTLVLQVNDTLTLKITG EADNILNFLSSLEGSMNTGFNN*(1402)VTSTLSAVENNILGKITDTSNLLSSKIDNTLSTLQDLI TSTSNDLKNSISSAKNDIVSSLSSKVDSSTQTLSTKLDDLKSAQESNTNSINNNIMLFGA ASLILLIVTIGLVGYRLIARRRVG
Sequence Around Glycosites (21 AA) SVKAGTYNFYNITPTTQTVEV
KVLFNPRWFYNGTYYIEHDKL
NTFTTEMWPFNTTIEVIGWPT
TNGYNMTNLYNLTIPFLPYGN
LLEGTLLSVNNRTVFTVHMGI
ADRLEMIINDNVSTILASIGN
LEGSMNTGFNNVTSTLSAVEN
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Technique(s) used for Glycosylation DetectionCarbohydrate analysis using trifluoroacetic acid and GC/MS (gas chromatography/mass spectrometry).
Technique(s) used for Glycosylated Residue(s) Detection Edman degradation
Glycan Information
Glycan Annotation Carbohydrates present: glucose, mannose, and glucosamine. Both N and O linked glycans present.
β-elimination revealed that at least 15 carbohydrate chains are bound to Ser or Thr. Two molecules of 150 KDa proteases are associated with glycosylated tetrabrachion polypeptides (85 KDa & 92KDa) and for this tetrabrachion-protease complex overall carbohydrate content is 38%.
Protein Glycosylation linked (PGL) gene(s)
Characterized Accessory Gene(s)Information currently not available with us.
Literature
Year of Identification1995
Year of Identification Month Wise1995.01.01
Year of Validation 1996
ReferenceÖzbek, S., Müller, J.F., Figgemeier, E. and Stetefeld, J., 2005. Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB). Acta Crystallographica Section D: Biological Crystallography, 61(4), pp.477-480.
Corresponding Author Jörg Stetefeld
ContactDepartment of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrase 70, CH-4056 Basel, Switzerland.
ReferenceStetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Engel, J. and Kammerer, R.A., 2000. Crystal structure of a naturally occurring parallel right-handed coiled coil tetramer. Nature structural biology, 7(9), pp.772-776.
Corresponding Author Jörg Stetefeld
ContactDepartment of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrase 70, CH-4056 Basel, Switzerland.
ReferencePeters, J., Baumeister, W. and Lupas, A., 1996. Hyperthermostable Surface Layer Protein Tetrabrachion from the ArchaebacteriumStaphylothermus marinus: Evidence for the Presence of a Right-handed Coiled Coil Derived from the Primary Structure. Journal of molecular biology, 257(5), pp.1031-1041.
Corresponding Author Jurgen Peters
ContactMax-Planck-Institut für Biochemie, Martinsried, Germany.
ReferencePeters, J., Nitsch, M., Kühlmorgen, B., Golbik, R., Lupas, A., Kellermann, J., Engelhardt, H., Pfander, J.P., Müller, S., Goldie, K. and Engel, A., 1995. Tetrabrachion: a filamentous archaebacterial surface protein assembly of unusual structure and extreme stability. Journal of molecular biology, 245(4), pp.385-401.
Corresponding Author Jurgen Peters
ContactMax-Planck-Institut für Biochemie, Martinsried, Germany.