ProGP1314(S-layer Protein, SLG)
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| ProGP ID | ProGP1314(S-layer Protein, SLG) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Methanothermococcus thermolithotrophicus |
| Domain | Archaea |
| Classification | Phylum : Euryarchaeota Class : Methanococci Orders : Methanococcales Family : Methanococcaceae Genus : Methanococcus Species : thermolithotrophicus |
| Taxonomic ID (NCBI) | 2186 |
| Genome Information | |
| GenBank | AJ308554.2 |
| Organism Additional Information | It is a thermophilic methanogen |
| Gene Information | |
| Gene Name | slg |
| NCBI Gene ID | AJ308554 |
| Protein Information | |
| Protein Name | Surface layer protein |
| UniProtKB/SwissProt ID | Q8X235 |
| NCBI RefSeq | CAC83952.2 |
| UniProtKB Sequence | ">sp|Q8X235|CSG_METTL S-layer protein OS=Methanothermococcus thermolithotrophicus OX=2186 GN=slmt1 PE=1 SV=1 MAMSLKKIGAIAVGGAMVASALASGVMAATTSGDVAGFMKNAIKEDGTPNVDIVVGSGAA VMDVVSAADVAAKIGSMAYKTGVVEDRSAVVKVSAKAESDDVNIFTLNATNEDIALVAAA DSDYAKGFINGSDQLKVTDQLASGSIQDVNDADFNATSLGDVSTMLKVPDIDPSDWYSND DDAGEVVFTRIVYDSDKLSIDEDQILYASIAYKNDEDVFNDNNTVTLKPGMRIPFLGEEY AVVKIDDEDDIIYLGKEAKDGVLKEGEXFAVGNGYEVKIASILKSGDTSTEYSVNVQILK DGKVVKEKTDTVGGSSSAQLKLAYKDVGVVVNDAWEDIAGTTGYAEVLITKDTKALELGE EYIPDWEAYAALNNSDKLEIKKDITESDKANIIGIALKYVGDKKKKLGDRDEVDIANYLK LVFDDEDKNDVLKVKFLMDESKEVTLDIGQKATVLNAEVRLKDILADAQQSVKLTAPIAK LDSEVSLDTADKNLILVGGPVVNALTKELVDAGKVAIDNTSPATLAVVEGAANGNDVLVV AGGDREATREAAKALLEMI" |
| Subcellular Location | Surface layer |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N107, N129, N221, N372 |
| Technique(s) used for Glycosylation Detection | SDS-PAGE, LC-MS/MS analysis |
| Glycan Information | |
| Glycan Annotation | 3-O-methyl-5-C-methyl-4-O-acetyl-β-galactopyranosyluronic acid, α-DDHep3,6OMe2, α-GalNAcA3OMe, α-GalA, α-GalAN, β-Man, β-Man |
| Technique(s) used for Glycan Identification | LC-MS/MS analysis |
| Literature | |
| Year of Identification | 2020 |
| Reference | Kelly, J.F., Vinogradov, E., Stupak, J., Robotham, A.C., Logan, S.M., Berezuk, A., Khursigara, C.M. and Jarrell, K.F., 2020. Identification of a novel N-linked glycan on the archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus. Journal of Biological Chemistry, 295(43), pp.14618-14629. |
| Corresponding Author | John F. Kelly Ken F. Jarrell |
| Contact | Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, Ontario, Canada Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, Ontario, Canada |