| Technique(s) used for Glycosylation Detection | Concanavalin A (ConA)-binding |
| Technique(s) used for Glycosylated Residue(s) Detection | LC-ESI-MS/MS (liquid chromatography-electrospray-ionization-tandem mass spectrometry) and site-directed mutagenesis (Thr to Ala) |
| Protein Glycosylation- Implication | Glycosylation of this enzyme modulates proteolytic cleavage within the unstructured SodC N-terminus and influences SodC localization and stability. |
| Glycan Information |
| Glycan Annotation | Linkages: αMan-Thr/Ser.
Variable modifications with one to three hexose (mannose) units on each site. The total number of hexose units present is in the range of 6-10. The most dominant SodC glycoform was modified with nine hexose units. |
| Technique(s) used for Glycan Identification | ESI-MS/MS (electrospray ionization tandem mass spctrometry) |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | Protein O-mannosyltransferase |
| OST ProGT ID | ProGT12 |
| Characterized Accessory Gene(s) | Polyprenol-phosphate-mannose (PPM) synthase, Ppm1, is present. A second type of Ppm synthase (Rv3779 gene product) exclusive to slow-growing mycobacteria, is a membrane glycosyltransferase. It mannosylates polyprenyl-phosphates directly from GDP-mannose. |
| Accessory Gene(s)Progt ID | ProGT10.1 |
| Additional Comment | Sec-mediated translocation influences the O-mannosylation. Ppm1 does not discriminate between polyprenol substrates with variable chain lengths and saturation of the isoprene units. |
| Literature |
| Year of Identification | 2000 |
| Year of Identification Month Wise | 2000.5.19 |
| Year of Validation | 2009 |
| Reference | Sartain, M.J. and Belisle, J.T., 2009. N-Terminal clustering of the O-glycosylation sites in the Mycobacterium tuberculosis lipoprotein SodC. Glycobiology, 19(1), pp.38-51. |
| Corresponding Author | John T Belisle |
| Contact | Department of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, CO 80523, USA. |
| Reference | Scherman, H., Kaur, D., Pham, H., Škovierová, H., Jackson, M. and Brennan, P.J., 2009. Identification of a polyprenylphosphomannosyl synthase involved in the synthesis of mycobacterial mannosides. Journal of bacteriology, 191(21), pp.6769-6772. |
| Corresponding Author | Hataichanok Scherman |
| Contact | Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-1682, USA. |
| Reference | Spagnolo, L., Törö, I., D'orazio, M., O'Neill, P., Pedersen, J.Z., Carugo, O., Rotilio, G., Battistoni, A. and Djinović-Carugo, K., 2004. Unique features of the sodC-encoded superoxide dismutase from Mycobacterium tuberculosis, a fully functional copper-containing enzyme lacking zinc in the active site. Journal of Biological Chemistry, 279(32), pp.33447-33455. |
| Corresponding Author | Kristina Djinovi?-Carugo
Andrea Battistoni
|
| Contact | Structural Biology Laboratory, ELETTRA, Sincrotrone Trieste, Italy.
Department of Biology, University of Rome Tor Vergata and Istituto Nazionale per la Fisica della Materia, Via della Ricerca Scientifica, 00133 Rome, Italy |
| Reference | Herrmann, J.L., Delahay, R., Gallagher, A., Robertson, B. and Young, D., 2000. Analysis of post-translational modification of mycobacterial proteins using a cassette expression system. Febs Letters, 473(3), pp.358-362. |
| Corresponding Author | Jean Louis Herrmann |
| Contact | Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St. Mary's Campus, Norfolk Place, W2 1PG, London, UK. jlh@chu-stlouis.fr |
