ProGP371 (Putative secreted protease)
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ProGP ID | ProGP371 (Putative secreted protease) |
Validation Status | Characterized |
Organism Information | |
Organism Name | Campylobacter jejuni HB93-13 |
Domain | Bacteria |
Classification | Phylum : Proteobacteria Class : Epsilonproteobacteria Orders : Campylobacterales Family : Campylobacteraceae Genus : Campylobacter Species : jejuni Subspecies : jejuni Strain : HB93-13 |
Taxonomic ID (NCBI) | 192222 |
Genome Information | |
GenBank | NC_002163.1 |
EMBL | AL111168 |
Organism Additional Information | Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity. |
Gene Information | |
Gene Name | cj0511 |
NCBI Gene ID | 904840 |
GenBank Gene Sequence | NC_002163.1. |
Protein Information | |
Protein Name | Putative secreted protease |
UniProtKB/SwissProt ID | Q0PB04 |
NCBI RefSeq | WP_002856815.1 |
EMBL-CDS | CAL34658.1 |
UniProtKB Sequence | >tr|Q0PB04|Q0PB04_CAMJE Putative secreted protease OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0511 PE=3 SV=1 MMELILKTKRFFAGLAGFATTFILCLFLTSHLQAKVDQKEEQVQKRLEALDKLTKTLAIV EQYYVDDQNISDLVDKSLSGLLSNLDAHSSFLNEKDFNDMKIQTNGEFGGLGITVGMKDG ALTVVSPIEGTPADKAGIKSGDIILKINDEATLGINLNDAVDKMRGKPKTQITLTIFRKG ATKPFDVTLTREIIKIESVYAKMIENENILYLRVTNFDKNVVDVASKELKKYPNVKGVIL DLRNNPGGLLNQAIGLVNLFVDKGVIVSQKGRIASENQEYKADPKNKISNASLVVLVNGG SASASEIVSGALQDLKRGVIVGENTFGKGSVQQIIPINKTEALRLTIARYYLPSGRTIQA VGVKPDIEVFPGKVNTQEDGFSIKESDLKQHLESELEKIDKNKKEDKQENKDNKNLISQK QINDDAQLKSAIDTIKILNIKQGQ |
Sequence length | 444 AA |
Glycosylation Status | |
Glycosylation Type | N- (Asn) linked |
Experimentally Validated Glycosite(s) in Full Length Protein | N69 |
Glycosite(s) Annotated Protein Sequence | >tr|Q0PB04|Q0PB04_CAMJE Putative secreted protease OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0511 PE=3 SV=1 MMELILKTKRFFAGLAGFATTFILCLFLTSHLQAKVDQKEEQVQKRLEALDKLTKTLAIV EQYYVDDQN*(69)ISDLVDKSLSGLLSNLDAHSSFLNEKDFNDMKIQTNGEFGGLGITVGMKDG ALTVVSPIEGTPADKAGIKSGDIILKINDEATLGINLNDAVDKMRGKPKTQITLTIFRKG ATKPFDVTLTREIIKIESVYAKMIENENILYLRVTNFDKNVVDVASKELKKYPNVKGVIL DLRNNPGGLLNQAIGLVNLFVDKGVIVSQKGRIASENQEYKADPKNKISNASLVVLVNGG SASASEIVSGALQDLKRGVIVGENTFGKGSVQQIIPINKTEALRLTIARYYLPSGRTIQA VGVKPDIEVFPGKVNTQEDGFSIKESDLKQHLESELEKIDKNKKEDKQENKDNKNLISQK QINDDAQLKSAIDTIKILNIKQGQ |
Sequence Around Glycosites (21 AA) | IVEQYYVDDQNISDLVDKSLS |
Technique(s) used for Glycosylation Detection | Soybean Lectin Affinity Enrichment and ZIC-HILIC enrichment |
Technique(s) used for Glycosylated Residue(s) Detection | Reversed Phase LC-Tandem CID/HCD-MS and ESI (CID)-MS/MS after enrichment using graphite microcolumns |
Glycan Information | |
Glycan Annotation | Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glcβ1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac-β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose) |
BCSDB ID | 20059 |
GlyTouCan | G38348FF |
Technique(s) used for Glycan Identification | Reversed Phase LC-Tandem CID/HCD-MS |
Literature | |
Year of Identification | 2011 |
Year of Identification Month Wise | 2011.2.10 |
Year of Validation | 2011 |
Reference | Scott, N.E., Parker, B.L., Connolly, A.M., Paulech, J., Edwards, A.V., Crossett, B., Falconer, L., Kolarich, D., Djordjevic, S.P., Højrup, P. and Packer, N.H., 2011. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Molecular & cellular proteomics, 10(2), pp.S1-S18. |
Corresponding Author | Stuart J Cordwell |
Contact | School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia |