ProGP371 (Putative secreted protease)

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ProGP ID ProGP371 (Putative secreted protease)
Validation Status Characterized
Organism Information
Organism NameCampylobacter jejuni HB93-13
Domain Bacteria
Classification Phylum : Proteobacteria
Class : Epsilonproteobacteria
Orders : Campylobacterales
Family : Campylobacteraceae
Genus : Campylobacter
Species : jejuni
Subspecies : jejuni
Strain : HB93-13
Taxonomic ID (NCBI) 192222
Genome Information
GenBank NC_002163.1
EMBL AL111168
Organism Additional Information Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity.
Gene Information
Gene Namecj0511
NCBI Gene ID 904840
GenBank Gene Sequence NC_002163.1.
Protein Information
Protein NamePutative secreted protease
UniProtKB/SwissProt ID Q0PB04
NCBI RefSeq WP_002856815.1
EMBL-CDSCAL34658.1
UniProtKB Sequence >tr|Q0PB04|Q0PB04_CAMJE Putative secreted protease OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0511 PE=3 SV=1 MMELILKTKRFFAGLAGFATTFILCLFLTSHLQAKVDQKEEQVQKRLEALDKLTKTLAIV EQYYVDDQNISDLVDKSLSGLLSNLDAHSSFLNEKDFNDMKIQTNGEFGGLGITVGMKDG ALTVVSPIEGTPADKAGIKSGDIILKINDEATLGINLNDAVDKMRGKPKTQITLTIFRKG ATKPFDVTLTREIIKIESVYAKMIENENILYLRVTNFDKNVVDVASKELKKYPNVKGVIL DLRNNPGGLLNQAIGLVNLFVDKGVIVSQKGRIASENQEYKADPKNKISNASLVVLVNGG SASASEIVSGALQDLKRGVIVGENTFGKGSVQQIIPINKTEALRLTIARYYLPSGRTIQA VGVKPDIEVFPGKVNTQEDGFSIKESDLKQHLESELEKIDKNKKEDKQENKDNKNLISQK QINDDAQLKSAIDTIKILNIKQGQ
Sequence length 444 AA
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN69
Glycosite(s) Annotated Protein Sequence >tr|Q0PB04|Q0PB04_CAMJE Putative secreted protease OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0511 PE=3 SV=1 MMELILKTKRFFAGLAGFATTFILCLFLTSHLQAKVDQKEEQVQKRLEALDKLTKTLAIV EQYYVDDQN*(69)ISDLVDKSLSGLLSNLDAHSSFLNEKDFNDMKIQTNGEFGGLGITVGMKDG ALTVVSPIEGTPADKAGIKSGDIILKINDEATLGINLNDAVDKMRGKPKTQITLTIFRKG ATKPFDVTLTREIIKIESVYAKMIENENILYLRVTNFDKNVVDVASKELKKYPNVKGVIL DLRNNPGGLLNQAIGLVNLFVDKGVIVSQKGRIASENQEYKADPKNKISNASLVVLVNGG SASASEIVSGALQDLKRGVIVGENTFGKGSVQQIIPINKTEALRLTIARYYLPSGRTIQA VGVKPDIEVFPGKVNTQEDGFSIKESDLKQHLESELEKIDKNKKEDKQENKDNKNLISQK QINDDAQLKSAIDTIKILNIKQGQ
Sequence Around Glycosites (21 AA) IVEQYYVDDQNISDLVDKSLS
Technique(s) used for Glycosylation DetectionSoybean Lectin Affinity Enrichment and ZIC-HILIC enrichment
Technique(s) used for Glycosylated Residue(s) Detection Reversed Phase LC-Tandem CID/HCD-MS and ESI (CID)-MS/MS after enrichment using graphite microcolumns
Glycan Information
Glycan Annotation Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glcβ1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac-β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose)
BCSDB ID 20059
GlyTouCan G38348FF
Technique(s) used for Glycan Identification Reversed Phase LC-Tandem CID/HCD-MS
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.10
Year of Validation 2011
ReferenceScott, N.E., Parker, B.L., Connolly, A.M., Paulech, J., Edwards, A.V., Crossett, B., Falconer, L., Kolarich, D., Djordjevic, S.P., Højrup, P. and Packer, N.H., 2011. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Molecular & cellular proteomics, 10(2), pp.S1-S18.
Corresponding Author Stuart J Cordwell
ContactSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia