ProGP432 (Hypothetical protein)

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ProGP ID ProGP432 (Hypothetical protein)
Validation Status Characterized
Organism Information
Organism NameSulfolobus solfataricus P2 (DSM 1617)
Domain Archaea
Classification Phylum : Crenarchaeota
Class : Thermoprotei
Orders : sulfolobales
Family : Sulfolobaceae
Genus : Sulfolobus
Species : solfataricus
Strain : P2 (DSM 1617)
Taxonomic ID (NCBI) 273057
Genome Information
GenBank AE006641
EMBL AE006641.1
Gene Information
Gene NameSSO2552
NCBI Gene ID 27428852
GenBank Gene Sequence NZ_LT549890.1
Protein Information
Protein NameConserved hypothetical protein
UniProtKB/SwissProt ID Q97VR1
NCBI RefSeq WP_009990124.1
EMBL-CDSAAK42681.1
UniProtKB Sequence >tr|Q97VR1|Q97VR1_SULSO Uncharacterized protein OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=SSO2552 PE=4 SV=1 MRKNIALILLFSILAGIIVVPISSSQTSSSISHPLILGNISVLNSGKIPYDPTYYSFEAY QIHPPNVTPVVIRIATNAVFNNSGLTPYIVHVNIPKGNYSMEILNVSIAESNGAQYDRPV YIFANGVPIFWGSTQEFFNSTAETDVTMFENLLSGNVTFQLVLENFYDAKIGITGIYKMN VTLYLYPGNPPKGLPNYFIPLFLNNHNYSYIILNPLNDYISQNVTIPNGTYRMTLLLYEE GGGLDEFWYANEPATREIQVFYDNRLVGVVNPYQTIYTGGIDLFWWKPVTSINTLSFHSP YIIDLTPLLAISLPNNTIAVTVTNLETALQLTGTAAYDWDIAGVLMLWVNESNPLVSAKL LTAYNRFIDSSPIFNSGLVGEYYQEGGAYLLNYSAILQFKDGIEYSDVVQQGRFYAYQTF NALYEKAYLGEKFMEYASERGSLYNATLYYNIYYPIFMQFSVFEAPISNPHVIPFNLSYA QNGTLDLWLYYNYTNIFDKQNLTIRTMENVSAVGGFSGIIEVINRYGGAVLVSITSNNAV TAKNLINYILLNGNGYKEIFSAKGLQNSTSHFAGYYIYYKVQFIPITNGDPPNAHSEFLI EPIHFLRIIV
Sequence length 610 AA
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN39
Glycosite(s) Annotated Protein Sequence >tr|Q97VR1|Q97VR1_SULSO Uncharacterized protein OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=SSO2552 PE=4 SV=1 MRKNIALILLFSILAGIIVVPISSSQTSSSISHPLILGN*(39)ISVLNSGKIPYDPTYYSFEAY QIHPPNVTPVVIRIATNAVFNNSGLTPYIVHVNIPKGNYSMEILNVSIAESNGAQYDRPV YIFANGVPIFWGSTQEFFNSTAETDVTMFENLLSGNVTFQLVLENFYDAKIGITGIYKMN VTLYLYPGNPPKGLPNYFIPLFLNNHNYSYIILNPLNDYISQNVTIPNGTYRMTLLLYEE GGGLDEFWYANEPATREIQVFYDNRLVGVVNPYQTIYTGGIDLFWWKPVTSINTLSFHSP YIIDLTPLLAISLPNNTIAVTVTNLETALQLTGTAAYDWDIAGVLMLWVNESNPLVSAKL LTAYNRFIDSSPIFNSGLVGEYYQEGGAYLLNYSAILQFKDGIEYSDVVQQGRFYAYQTF NALYEKAYLGEKFMEYASERGSLYNATLYYNIYYPIFMQFSVFEAPISNPHVIPFNLSYA QNGTLDLWLYYNYTNIFDKQNLTIRTMENVSAVGGFSGIIEVINRYGGAVLVSITSNNAV TAKNLINYILLNGNGYKEIFSAKGLQNSTSHFAGYYIYYKVQFIPITNGDPPNAHSEFLI EPIHFLRIIV
Sequence Around Glycosites (21 AA) SSISHPLILGNISVLNSGKIP
Technique(s) used for Glycosylation DetectionConcanavalin A lectin affinity chromatography
Glycan Information
Glycan Annotation Branched sulfated heptasaccharide, Hex4(GlcNAc)2 plus sulfoquinovose where Hex is D-mannose and D-glucose.
Protein Glycosylation linked (PGL) gene(s)
Additional CommentThe glycan structure is by far the most complex one known in archaea till now.
Literature
Year of Identification2013
Year of Identification Month Wise2013.6.1
Year of Validation 2013
ReferenceGianna Palmieri, Marco Balestrieri, Jasna Peter-Katalinić, Gottfried Pohlentz, Mosè Rossi, Immacolata Fiume, and Gabriella Pocsfalvi (2013) Surface-exposed glycoproteins of hyperthermophilic Sulfolobus solfataricus P2 show a common N-glycosylation profile. J. Proteome Res., 12, 2779−2790.
Corresponding Author Gabriella Pocsfalvi
ContactInstitute of Protein Biochemistry, National Research Council of Italy, Napoli, Italy.