ProGP638 (Excisionase)

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ProGP ID ProGP638 (Excisionase)
Validation Status Uncharacterized
Organism Information
Organism NamePyrobaculum calidifontis JCM 11548
Domain Archaea
Classification Phylum : Crenarchaeota
Class : Thermoprotei
Orders : Thermoproteales
Family : Thermoproteaceae
Genus : Pyrobaculum
Species : calidifontis
Strain : JCM 11548
Taxonomic ID (NCBI) 410359
Genome Information
GenBank CP000561.1
EMBL CP000561
Organism Additional Information Pyrobaculum calidifontis is a hyperthermophilic archaeon that belongs to the phylum Crenarchaeota. In contrast to the phylum Euryarchaeota, only the N-glycan structure of the genus Sulfolobus is known in Crenarchaeota.
Gene Information
Gene NamePcal_0785
Protein Information
Protein NameExcisionase
UniProtKB/SwissProt ID A3MU94
NCBI RefSeq ABO08211.1.
EMBL-CDSABO08211.1
UniProtKB Sequence >tr|A3MU94|A3MU94_PYRCJ Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase and related enzyme-like protein OS=Pyrobaculum calidifontis (strain JCM 11548 / VA1) GN=Pcal_0785 PE=4 SV=1 MRLVVLLTLAAFLVLAVGPIRLEVQSVLVSPVNTTLIVDYVKAAKKAVYMEVYVFTYKPL ADALASAAKRGVDVVVVLSARVYGGVPQSAKEIASYLEANGVKVKWNSDFPNVHTKLYVI DNETVIIGNINPTASGFTKNKGIMLVIKNATLARQLATIVLNDYRGVYPRYNYPGVLVSP INSQDGLLWILSQPGVRYIAMEQIYVDSGLVPYIVNGPYYAVVARTDADINTAVDEDLVA KIIVVGNYVYVGSINIGYYSIQRNREVGLLIENPGLAQRLKTLVMKWYQEAGGKVETTTE ASTATAPPSTSTTPKAQNAGRSYSLDVLIWLAIIIIAILFVIWANRRRP
Sequence length 349 AA
Subcellular LocationIntegral component of membrane
Function Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthase and related enzyme-like protein
Glycosylation Status
Glycosylation Type N- (Asn) linked
Technique(s) used for Glycosylation DetectionConA(Concanavalin A) lectin affinity chromatography and detected by periodic acid-Schiff (PAS) staining and Coomassie Brilliant Blue (CBB) staining on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gels, RP-HPLC
Technique(s) used for Glycosylated Residue(s) Detection Normal-phase-HPLC-MS/MS analysis, LC-ESI-MS/MS analysis
Glycan Information
Glycan Annotation Pentasaccharide (α-Man-(1-3)-(α-Man-(1-6)-)α-Man-(1-4)-β-GlcANAc3NAc-(1-4)-β-GlcNAc3NAc)
GlyTouCan G06484YZ G62526ZK
Literature
Year of Identification2017
Year of Validation 2017
ReferenceFujinami, D., Taguchi, Y. and Kohda, D., 2017. Asn-linked oligosaccharide chain of a crenarchaeon, Pyrobaculum calidifontis, is reminiscent of the eukaryotic high-mannose-type glycan. Glycobiology, 27(8), pp.701-712.
Corresponding Author Daisuke Kohda
Contact1. Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
2. Research Center for Advanced Immunology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
3. Research Center for Live-Protein Dynamics, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.