ProGP662 (30S ribosomal protein S2)

Home -> ProGPdb -> Search ProGP -> Display data

ProGP ID ProGP662 (30S ribosomal protein S2)
Validation Status Uncharacterized
Organism Information
Organism NamePyrobaculum calidifontis JCM 11548
Domain Archaea
Classification Phylum : Crenarchaeota
Class : Thermoprotei
Orders : Thermoproteales
Family : Thermoproteaceae
Genus : Pyrobaculum
Species : calidifontis
Strain : JCM 11548
Taxonomic ID (NCBI) 410359
Genome Information
GenBank CP000561.1
EMBL CP000561
Organism Additional Information Pyrobaculum calidifontis is a hyperthermophilic archaeon that belongs to the phylum Crenarchaeota. In contrast to the phylum Euryarchaeota, only the N-glycan structure of the genus Sulfolobus is known in Crenarchaeota.
Gene Information
Gene NamePcal_0027
Protein Information
Protein Name30S ribosomal protein S2
UniProtKB/SwissProt ID A3MS50
NCBI RefSeq ABO07467.1.
EMBL-CDSABO07467.1
UniProtKB Sequence >sp|A3MS50|RS2_PYRCJ 30S ribosomal protein S2 OS=Pyrobaculum calidifontis (strain JCM 11548 / VA1) GN=rps2 PE=3 SV=1 MAEEAQYEYLVPLEKYLSAGVRLGTRLSNKYLEERGFIYAVRPDGLRIFDIKKIDERLRI AAKLIARYPPDRVLVHTTRPYGFKPVQMFCKFVGCKALTGRFIPGMLTNPNLSNYLEVDL LFVVDPRLDSQAVAEAAKMGIPVVALVDTDTPHQYIDFMIPCNNKGRKSLALIFWVLARQ VLRERGELKPDQDLPVPPEEFETRLVQT
Sequence length 208 AA
Subcellular LocationSmall ribosomal subunit
Function SSU ribosomal protein S2P
Glycosylation Status
Glycosylation Type N- (Asn) linked
Technique(s) used for Glycosylation DetectionConA(Concanavalin A) lectin affinity chromatography and detected by periodic acid-Schiff (PAS) staining and Coomassie Brilliant Blue (CBB) staining on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gels, RP-HPLC
Technique(s) used for Glycosylated Residue(s) Detection Normal-phase-HPLC-MS/MS analysis, LC-ESI-MS/MS analysis
Glycan Information
Glycan Annotation Pentasaccharide (α-Man-(1-3)-(α-Man-(1-6)-)α-Man-(1-4)-β-GlcANAc3NAc-(1-4)-β-GlcNAc3NAc)
GlyTouCan G06484YZ G62526ZK
Literature
Year of Identification2017
Year of Validation 2017
ReferenceFujinami, D., Taguchi, Y. and Kohda, D., 2017. Asn-linked oligosaccharide chain of a crenarchaeon, Pyrobaculum calidifontis, is reminiscent of the eukaryotic high-mannose-type glycan. Glycobiology, 27(8), pp.701-712.
Corresponding Author Daisuke Kohda
Contact1. Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
2. Research Center for Advanced Immunology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
3. Research Center for Live-Protein Dynamics, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.