ProGP700 (SlpA protein)
Home -> ProGPdb -> Search ProGP -> Display data
| ProGP ID | ProGP700 (SlpA protein) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Clostridium difficile SLCT-11 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Clostridia Orders : Eubacteriales Family : Peptostreptococcaceae Genus : Clostridium Species : difficile Strain : SLCT-11 |
| Taxonomic ID (NCBI) | 1496 |
| Genome Information | |
| GenBank | NZ_CP019870.1 |
| Gene Information | |
| Gene Name | orf |
| Protein Information | |
| Protein Name | SlpA protein |
| NCBI RefSeq | WP_078051019.1 |
| UniProtKB Sequence | >WP_078051019.1 S-layer protein SlpA [Clostridioides difficile] MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLLKELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGKYVDFKITYGAKAEVPAASLSAADIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNKVIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASLSDVDAITLDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAVEKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVTIFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQTVAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAPLLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLKVTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVVDGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNAEVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPAPIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM |
| Subcellular Location | S-layer of cell wall |
| Function | Sporulation, cell length, and biofilm formation |
| Glycosylation Status | |
| Glycosylation Type | O- (Thr) linked |
| Experimentally Predicted Glycosites | T-38 |
| Experimentally Validated Glycosite(s) in Full Length Protein | T-38 |
| Experimentally Validated Glycosite(s ) in Mature Protein | >WP_078051019.1 S-layer protein SlpA [Clostridioides difficile] MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYT*(34)VVQDKYEKLLKELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGKYVDFKITYGAKAEVPAASLSAADIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNKVIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASLSDVDAITLDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAVEKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVTIFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQTVAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAPLLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLKVTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVVDGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNAEVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPAPIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM |
| Glycosite(s) Annotated Protein Sequence | ADTTVKEEGYTVVQDKYEKLL |
| Technique(s) used for Glycosylation Detection | Glycoproteomic and glycomic MS analyses |
| Technique(s) used for Glycosylated Residue(s) Detection | MS analysis |
| Protein Glycosylation- Implication | The glycosylation is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer. |
| Glycan Information | |
| Glycan Annotation | Tetrasaccharide -4-α-Rha-3-α-Rha-3-α-Rha-3-β; pentasaccharide -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; non reducing 2,3 cyclophosphoryl-rhamnose |
| Technique(s) used for Glycan Identification | MS and NMR |
| Literature | |
| Year of Identification | 2018 |
| Reference | Richards, E., Bouché, L., Panico, M., Arbeloa, A., Vinogradov, E., Morris, H., Wren, B., Logan, S.M., Dell, A. and Fairweather, N.F., 2018. The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology. Journal of Biological Chemistry, 293(47), pp.18123-18137. |
| Corresponding Author | Anne Dell Neil F Fairweather |
| Contact | Dept. of Life Sciences, SirErnst Chain Bldg., Imperial College London, London SW7 2AZ, UK |