ProGT100 (PglB)
ProGT ID | ProGT100 (PglB) |
Organism Information | |
Organism Name | Sulfurimonas denitrificans DSM 1251 |
Clinical Implication | Non-pathogenic |
Domain | Bacteria |
Classification | Phylum : Proteobacteria Class : EpsilonProteobacteria Orders : Campylobacterales Family : Thiovulaceae Genus : Sulfurimonas Species : denitrificans Strain : DSM 1251 |
Taxonomic ID (NCBI) | 326298 |
Genome Information | |
Gene Bank | CP000153 |
EMBL | CP000153 |
Gene Information | |
Gene Name | Suden_1474 |
Protein information | |
Protein Name | PglB |
UniProtKB/ SwissProt ID | Q30QI0 |
NCBI Ref Seq | WP_011373103.1 |
UniProtKB Sequence | >tr|Q30QI0|Q30QI0_SULDN Oligosaccharyl transferase, STT3 subunit OS=Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) GN=Suden_1474 PE=4 SV=1 MLTFTKETKVTLLYIALAFLFSVAIRMIWFYQFVDYEPFRFNAQLMINTNDGYYWAEGAR DILAGVHQDNDLSAVDLAASQLSALFAYILPFSFESVILFMPVFLGSLIVIPIILIAKSI DNLEMGFIAALLASIAHSYYNRTMIGYYDTDMLNIVLPMFLLWSIIWAVRTNQDKYLLIT AVDILIYRWWYPQSYSLESAFFALILLYTLLYERKNSYNYKLLAIMMFAMVGFEGYIRAI FVLIAFYMYKQEKYDRYVHSVLAFSIVLFFVSGGFNPIWEQLSGYVFKDAISVGQEGLKL HFYSVKQTIREAANIPFITFANRISGHVITFLASIIGYIYLVYKHKVMLFGLPLIGLGFL AYVGGLRFTIYAVPVLAFGVAFLISEIARFMPNRRVKVFSMSALTLLILLPNIVHIEAYR VPTVFNADEVKVLDMLRGKANREDYVVAWWDYGYPIRYYSDVKTLIDGGKHRGDVNFPVS FMLTNPQDVSAKLARLDVEYSENSFEDKNPTMFSNIEKMTKEYGFSDTNDFLLSLEGEIE LPKKTRDIYFYLPHRMLDIYPTVEIFSNLDLMNGNMKNRSFFYATKQFRQEQNILNLSQG VVVDLKEFSVNINNNKVPIKRVIQSAYDKNMKFTKEVELVNPNGELNLIFMSSYNTFLVV DEKTYNSVYIQLSVLQEYDKMLFEEVIITPHAKVYKLKI |
EMBL CDS | ABB44751.1 |
Sequence length | 699 AA |
Subcellular Location | Membrane (Integral component of membrane) |
String | 326298.Suden_1474 |
Additional Information | 1) It is able to transfer glycans to the acceptor sites, predominantly to the sites having a negatively charged residue in the ?2 position relative to the asparagine. |
Glycosyltransferase Information | |
Glycosylation Type | N- (Asn) linked |
CAZY Family | GT66 |
EC Number (BRENDA) | 2.4.1.- |
Mechanism of Glycan Transfer | En bloc |
Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
Donor Type | UndPP-Heptasaccharide |
Donor Specificity | Lipid linked sugars |
Glycan Information | |
Glycan transferred | Heptasaccharide |
Method of Glycan Indentification | LC-MS |
Experimental_strategies | In vivo and In vitro |
Acceptor Subtrate Information | |
Acceptor Substrate name | scFv13-R4 DQNAT |
Acceptor Substrate name | TAMRA-GDQNATAF |
Acceptor Substrate name | AcrA |
ProGPdb ID | ProGP217 |
Litrature | |
Year Of Validation | 2015 |
Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. and DeLisa, M.P., 2015. Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5(1), pp.1-13. |
Corresponding Author | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |