ProGT115 (GT1)

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ProGT ID ProGT115 (GT1)
ProGT Pathway
Organism Information
Organism NameClostridium difficile R20291
Clinical ImplicationPathogenic
DomainBacteria
Classification Phylum : Firmicutes
Class : Clostridia
Orders : Eubacteriales
Family : Peptostreptococcaceae
Genus : Clostridium
Species : difficle
Strain : R20291
Taxonomic ID (NCBI)645463
Genome Information
Gene BankFN545816
EMBLFN545816
Gene Information
Gene NameCDR20291_0241
Protein information
Protein NameGT1 
UniProtKB/ SwissProt IDC9YI48
NCBI Ref SeqCBE01877
UniProtKB Sequence>tr|C9YI48|C9YI48_PEPDR Putative glycosyltransferase OS=Peptoclostridium difficile (strain R20291) GN=CDR20291_0241 PE=4 SV=1 MDIEERVRLCFTPFFFYKYTFERKIYMLLSIVMIVKNEEKILEKTLKSLTTLRSSIESEL IIVDTGSTDDTINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSS TIVKFFKDGLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKN PIFNNVADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKY HEALYYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYY YIAQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSFYK NIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEIIYAYI IKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDIKKIRVY KAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCNDLDKFTIEF KHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKESEEFKDLKDS FIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKLDKADFMLKKALS LDVENEDTIYNIEYLKSLR
EMBL CDSCBE01877.1
Sequence length739 AA
Function in Native Organism 1) GT1 transfer GlcNAc to the flagella.
Glycosyltransferase Information
Glycosylation TypeO- (Ser/Thr) linked 
CAZY FamilyGT2
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferSequential
Donor TypeUDP-GlcNAc
Donor SpecificityNucleotide activated sugars
Accessory GT IDProGT115.1, ProGT115.2
Glycan Information
Glycan transferredMonosaccharide (GlcNAc) 
Method of Glycan IndentificationNano-LC-MS/MS
Experimental_strategiesIn vivo 
Acceptor Subtrate Information
Acceptor Substrate name FliC
ProGPdb ID ProGP298
Litrature
Year Of Validation2016 
Reference Valiente, E., Bouché, L., Hitchen, P., Faulds-Pain, A., Songane, M., Dawson, L.F., Donahue, E., Stabler, R.A., Panico, M., Morris, H.R. and Bajaj-Elliott, M., 2016. Role of glycosyltransferases modifying type B flagellin of emerging hypervirulent Clostridium difficile lineages and their impact on motility and biofilm formation. Journal of Biological Chemistry, 291(49), pp.25450-25461.

Corresponding AuthorDepartment of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.