ProGT13 (AglB)
| ProGT ID | ProGT13 (AglB) |
| Organism Information | |
| Organism Name | Methanococcus maripaludis (strain S2 / LL) |
| Clinical Implication | Non-pathogenic |
| Domain | Archaebacteria |
| Phylum | Euryarchaeota |
| Classification | Family: Methanococcaceae Order: Methanococcales Class: Methanococci or Methanothermea Division or phylum: "Euryarchaeota" |
| Taxonomic ID (NCBI) | 267377 |
| Genome Information | |
| Gene Bank | BX950229 |
| EMBL | BX950229 |
| Gene Information | |
| Gene Name | MMP1424 |
| NCBI Gene ID | 2761329 |
| Protein information | |
| Protein Name | AglB |
| UniProtKB/ SwissProt ID | Q6LXC8 |
| NCBI Ref Seq | WP_011171368.1 |
| UniProtKB Sequence | >tr|Q6LXC8|Q6LXC8_METMP Oligosaccharyl transferase, STT3 subunit OS=Methanococcus maripaludis (strain S2 / LL) GN=MMP1424 PE=4 SV=1 MGEFLNKVSDFFKKNEKIKIILILLFIGMMSFQIRAQTADMAFTDNSYLQDMFSDDNGRM YLTALDPYYYLRMTENYVNNDYSNVGETTVGIDGENIPYDTIQYAPPGREAGLVSALSIA TVLVYSVWNSIDSTVTIMNAAFWVPAIMSIFLGIPVFFIVRRNTASNIGGLVGALLLISS PSLLYKTSAGFSDTPIFEILPLLFIVWMIMEAIHEQENSKKSGIFGGIAAILIGLYPMMW SGWWYAFDITAGFLVLYTAYEYLTKSKNLKNVITTSLITLVGGAILVSLSTGLSGFINWI LSPIGFTVINEATKITGWPNVYMTVSELAIPTVTDIIENSVGNIWLLIAGISGILLSFVS FKHDKQKIDIKYALYLTLWLIATVYAATKGIRFVALMTPALAIGIGIFAGQIENIIKRYE KKVEYILYPVIGILSVITLIKYGGELFNILVPTTYVPIAVYLSIIALLVLAVYKIIDIIS EKEQAVKKVFGILLAFMLVFPSMAAAVPFYTAPTMNNGWMDSLSWIKSETPENSVVTCWW DNGHIYTWATRKMVTFDGGSQNTPRAYWVGHAFSTSDENLSVGILRMLATSGDSAYDDDS ILIKKTGSIKDTVDILNKILPLTRTEAKASLVNNYDLTDAEAEEVLDLTHPKVTNPDYLI TYNRMTSIASVWSMFGNWNFSLPASTENSDREMGYYQQLGGSAQDINGTTVVYIPLQETD SYRVINILEITDSEIKSANAVIDSNNQTSMQSPNFHKLILKVNGNVYEQETNENGDYSEI VRLEKLSDGTYQVYAWVSSKNLEDSIYTKLHFLDGYGLEKISLEKESVDPTSYGIQPGFK VYSVDYGTDYLN |
| EMBL CDS | CAF30980.1 |
| Sequence length | 852 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) AglB is OSTase transfer the tetrasaccharide to the S-layer protein. |
| String | 267377.MMP1424. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18.11922. |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | Lipid linked sugars |
| Donor Specificity | DolP-Tetrasaccharide |
| Accessory GT ID | ProGT13.1 ProGT13.2 ProGT13.3 ProGT13.4 ProGT13.5 ProGT13.6 ProGT13.7 ProGT13.8 ProGT13.9 ProGT13.10 ProGT13.11 ProGT13.12 ProGT13.13 ProGT13.14 ProGT13.15 ProGT13.16 ProGT13.17 ProGT |
| Glycan Information | |
| Glycan transferred | Tetrasaccharide (N-acetylgalactosamine, di-acetylated glucuronic acid, an acetylated and acetamidino-modified mannuronic acid linked to threonine, and a novel terminal sugar [(5S)-2-acetamido-2,4-dideoxy-5-O-methyl-a-L-erythro-hexos-5-ulo-1,5-pyranose]) |
| Method of Glycan Indentification | Nano-LC-MS/MS |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | FlaB1 |
| ProGPdb ID | ProGP259 |
| Acceptor Substrate name | FlaB2 |
| ProGPdb ID | ProGP260 |
| Acceptor Substrate name | FlaB3 |
| ProGPdb ID | ProGP258 |
| Litrature | |
| Year Of Validation | 2006 |
| Reference | Siu, S., Robotham, A., Logan, S. M., Kelly, J. F., Uchida, K., Aizawa, S. I., & Jarrell, K. F. (2015). Evidence that biosynthesis of the second and third sugars of the archaellin tetrasaccharide in the archaeon Methanococcus maripaludis occurs by the same pathway used by Pseudomonas aeruginosa to make a di-N-acetylated sugar. Journal of bacteriology, JB-00040. |
| Authors | Siu, S., Robotham, A., Logan, S. M., Kelly, J. F., Uchida, K., Aizawa, S. I., & Jarrell, K. F. |
| Research groups | Department of Biomedical and Molecular Sciences, Queens University, Kingston, Ontario, Canada |
| Corresponding Author | Jarrell, K. F. |
| Contacts | Department of Biomedical and Molecular Sciences, Queens University, Kingston, Ontario, Canada |
| Reference | VanDyke, D. J., Wu, J., Logan, S. M., Kelly, J. F., Mizuno, S., Aizawa, S. I., & Jarrell, K. F. (2009). Identification of genes involved in the assembly and attachment of a novel flagellin N?linked tetrasaccharide important for motility in the archaeon Methanococcus maripaludis. Molecular microbiology, 72(3), 633-644. |
| Authors | VanDyke, D. J., Wu, J., Logan, S. M., Kelly, J. F., Mizuno, S., Aizawa, S. I., & Jarrell, K. F. |
| Research groups | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada |
| Corresponding Author | Jarrell, K. F. |
| Contacts | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada |