ProGT140 (Maf)

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ProGT ID ProGT140 (Maf)
Organism Information
Organism NameGeobacillus kaustophilus HTA426
Classification Phylum : Firmicutes
Class : Bacilli
Orders : Bacillales
Family : Bacillaceae
Genus : Geobacillus
Species : kaustophilus
Strain : HTA426
Taxonomic ID (NCBI)235909
Genome Information
Gene Information
Gene Namemaf
Protein information
Protein NameMaf 
NCBI Ref SeqBAD77414.1
Function in Native Organism It is sialyltransferase and transfer Neu5Ac to flagella
Additional Information D285A and N379A in Maf are important for its GT activities
Glycosyltransferase Information
Glycosylation TypeO- (Ser/Thr) linked 
Mechanism of Glycan TransferSequential
Donor TypeCMP-Neu5Ac
Donor SpecificityNucleotide activated sugars
Glycan Information
Glycan transferredMonosaccharide (Neu5Ac) 
Method of Glycan IndentificationLC–MS/MS and Western blotting
Experimental_strategiesIn vivo 
Acceptor Subtrate Information
Acceptor Substrate name GkFlaA1
ProGPdb ID ProGP1211 (GkFlaA1)
Acceptor Substrate name GkFlaA2
ProGPdb ID ProGP1212 (GkFlaA2)
Year Of Validation2021 
Reference Khairnar, A., Sunsunwal, S., Babu, P. and Ramya, T.N.C., 2021. Novel serine/threonine-O-glycosylation with N-acetylneuraminic acid and 3-deoxy-D-manno-octulosonic acid by bacterial flagellin glycosyltransferases. Glycobiology, 31(3), pp.288-306.

Corresponding AuthorDepartment of Protein Science and Engineering, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India