ProGT19 (Lgt1)
| ProGT ID | ProGT19 (Lgt1) |
| Organism Information | |
| Organism Name | Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Legionellales Family : Legionellaceae Genus : Legionella Species : pneumophila Subspecies : pneumophila Strain : Philadelphia 1 / ATCC 33152 / DSM 7513 |
| Taxonomic ID (NCBI) | 272624 |
| Genome Information | |
| Gene Bank | AE017354 |
| EMBL | AE017354 |
| Gene Information | |
| Gene Name | lgt1 (lpg1368) |
| NCBI Gene ID | 19832933 |
| Protein information | |
| Protein Name | Lgt1 |
| UniProtKB/ SwissProt ID | Q5ZVS2 |
| NCBI Ref Seq | WP_010947098.1 |
| UniProtKB Sequence | >tr|Q5ZVS2|Q5ZVS2_LEGPH Uncharacterized protein OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=lpg1368 PE=1 SV=1 MKARRDQQLSKLRMRFFSALNHTSEIDLQVLFNDLKSILTLDSIEHLKEGSVAYAIIQEL LKQDDAQNKIQSFLHGAIKNVIHPGVIKGLTPDEINWNVAKAYPKYYEHEEFPDVTFGGF KVRDSNEFKFKTNIQTSIWFSIKPDLFMPSKQQEALKRRREQYPGCEIRLIYSSSLLNAE ANRQMKAFARKQNISLIDIDSVKTDSPLYPLLKAELAHLGKGGNPAAASDLCRWIPELFN EGFYVDIDLPVDSSKIVEGHQITGGVPIMLNMGSIISEPIAPHHRRQEAVCMNTDIIAYS NDKRTQKMMDTVARYLKNIYDDPYTALKDTPLAQTAFFNKCQEERKSIFDLRKGLQDAFR SDSLLQLYDFLGADKFKEVFKLKEAQSKYINEHISEFSEKDLLLNLISDKPSEISQHTLD FVKAKAMYIDIAKEHYSAFYKPLVEEISGPGAIYNALGGAGSFTTTHRRLTGPMLPTTPP RVLQVFCDAHDKGPFVSDNIARWQTNVRDLGVLNREGLSWLPSVG |
| EMBL CDS | AAU27450.1 |
| Sequence length | 525 AA |
| Subcellular Location | Host cell plasma membrane |
| String | 272624.lpg1368. |
| Additional Information | 1) Lgt1 monoglycosylate eukaryotic EF1A results in inhibition of eukaryotic protein synthesis and this lead to the death of target cells. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser) linked |
| CAZY Family | GT88 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-Glc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Glc) |
| Method of Glycan Indentification | MS and NMR |
| Experimental_strategies | In vitro and In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | EF1A |
| Acceptor Substrate name | Hsp70 subfamily B suppressor 1 (Hbs1) |
| Litrature | |
| Year Of Validation | 2006 |
| Reference | Belyi, Y., Niggeweg, R., Opitz, B., Vogelsgesang, M., Hippenstiel, S., Wilm, M. and Aktories, K., 2006. Legionella pneumophila glucosyltransferase inhibits host elongation factor 1A. Proceedings of the National Academy of Sciences, 103(45), pp.16953-16958. |
| Corresponding Author | Gamaleya Research Institute, Ulitsa Gamalei 18, Moscow 123098, Russia. |
| Reference | Belyi, Y., Stahl, M., Sovkova, I., Kaden, P., Luy, B. and Aktories, K., 2009. Region of elongation factor 1A1 involved in substrate recognition by Legionella pneumophila glucosyltransferase Lgt1: identification of Lgt1 as a retaining glucosyltransferase. Journal of Biological Chemistry, 284(30), pp.20167-20174. |
| Corresponding Author | Gamaleya Research Institute, Ulitsa Gamalei 18, Moscow 123098, Russia. |
| Reference | Hurtado-Guerrero, R., Zusman, T., Pathak, S., Ibrahim, A.F., Shepherd, S., Prescott, A., Segal, G. and van Aalten, D.M., 2010. Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase. Biochemical Journal, 426(3), pp.281-292. |
| Corresponding Author | Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK. |