ProGT20 (GmaR)
| ProGT ID | ProGT20 (GmaR) |
| Organism Information | |
| Organism Name | Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Listeriaceae Genus : Listeria Species : monocytogenes Strain : ATCC BAA-679 / EGD-e |
| Taxonomic ID (NCBI) | 169963 |
| Genome Information | |
| Gene Bank | AL591976.1 |
| EMBL | AL591976 |
| Gene Information | |
| Gene Name | lmo0688 |
| NCBI Gene ID | 986281 |
| NCBI Reference Sequence | NC_003210.1 |
| Protein information | |
| Protein Name | GmaR |
| UniProtKB/ SwissProt ID | Q8Y949 |
| NCBI Ref Seq | NP_464215.1 |
| UniProtKB Sequence | >tr|Q8Y949|Q8Y949_LISMO Lmo0688 protein OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) GN=lmo0688 PE=4 SV=1 MRPLISICMIVKNEAHILRQSLASFRKFTEEIIIVDTGSTDETKEIAQEFTDFVYDFEWT GNFSDARNFAAKHATGKWILAIDADECLEEESYRKLEKQLKSPTEPIQMAQIISFTGEKG RVTTTNQMARVYKNDGTICFRGVIHEQLEAIDKRPIAAGVAEVKIYHYGYMSEIVEKQDK SDRNLRLLEKEVKNNKNSGFVHFNIGQEMNRLGNKKEALKEFSEAFRLRDHNHYIWAKLS AYHIAELLEQEKRYDESLAIIEEARVIWPNVPEFPLKKANILYVNHQLEDAKEIYQSLLE NTAIDYQPIVLYEATNFMPHKMLGTIYLEEKDYTRAMTHFSKAYAENSSDYGVMFQMIML LSKFHQPKEIFAFMERHHFISSTETGLRLLSMTTQQGYAELSELIVQSLTDVYPPVAEAT EVKIATIRNVFPVISESAILFGVKEELIDAADLCLWHYENPQLPIENVMKNSDVGDIYDF IFENGPRISKKRYLFVLERAIALGKGEFADYLLALRNVYHDSINSHIADLFFQYDFADIA LDFYNIVDADEVTKQGYINLINYLVDADVLDEALAIAERGIDNFSTDFRFYLWAIKIDTE NRANRISEAMDEFPNNRYLAKLLDEVTMLQDTVTNNR |
| EMBL CDS | CAC98766.1. |
| Sequence length | 637 AA |
| Subcellular Location | Cytoplasm |
| Function in Native Organism | 1) GmaR is functioning as glycosyltransferase which transfers the GlcNAc to the flagella which are involved in motility. |
| String | 169963.lmo0688. |
| Additional Information | 1) GmaR is bifunctional O-GlcNAc transferase that transcriptionally regulates expression of its enzymatic substrate FlaA. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT2 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Method of Glycan Indentification | GC-MS and Western blot |
| Experimental_strategies | In vitro and In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | FlaA |
| ProGPdb ID | ProGP232 |
| Litrature | |
| Year Of Validation | 2006 |
| Reference | Shen, A., Kamp, H.D., Gründling, A. and Higgins, D.E., 2006. A bifunctional O-GlcNAc transferase governs flagellar motility through anti-repression. Genes & Development, 20(23), pp.3283-3295. |
| Corresponding Author | Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA. |
| Reference | Kamp, H.D. and Higgins, D.E., 2009. Transcriptional and post?transcriptional regulation of the GmaR antirepressor governs temperature?dependent control of flagellar motility in Listeria monocytogenes. Molecular microbiology, 74(2), pp.421-435. |
| Corresponding Author | Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA. |
| Reference | Kamp, H.D. and Higgins, D.E., 2011. A protein thermometer controls temperature-dependent transcription of flagellar motility genes in Listeria monocytogenes. PLoS pathogens, 7(8), p.e1002153. |
| Corresponding Author | Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA. |