ProGT27 (Lgt2)
| ProGT ID | ProGT27 (Lgt2) |
| Organism Information | |
| Organism Name | Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Legionellaceae Order: Legionellales Class: Gammaproteobacteria Phylum: Proteobacteria |
| Taxonomic ID (NCBI) | 272624 |
| Genome Information | |
| Gene Bank | AE017354 |
| EMBL | AE017354 |
| Gene Information | |
| Gene Name | lgt2 |
| Protein information | |
| Protein Name | Lgt2 |
| UniProtKB/ SwissProt ID | Q5ZRL9 |
| NCBI Ref Seq | WP_010948548.1 |
| UniProtKB Sequence | >tr|Q5ZRL9|Q5ZRL9_LEGPH Coiled-coil containing protein OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=legC8 PE=4 SV=1 MSEQYWRFKLMTEGGCNQNEATRLITVLKRKLKPFPEESINKLFENDNFCNRLSSYMAYG FGAAEEWIKKQQILSNIQPLTSEQRVDITPEKDTVSDNKPNIFGAAITFGKSPVVKLLKQ NAREICESILMDEPNLKQVEYIFRLLALQVQETYSGEQAEKLYECIRDKKPIPSKFEEIL LPIVNRIKENHTEILNESKRNHLGVTIQLNDPYSFSTKNSFCIWFSNNPNSAMPKKIKDI LEERAKQNAPGVTKLVYSRACLTKKENTNFVQWAKENGITLLDFDELKCQGEDLELWNLA QAELKAMREGKGGNPAAASDLVRWISGVIGDVPIAYVDADMPMLTGNKSIKSEEVYAGHP VLLNMGSALVKDGVNLPMENVAFNTDIINFTGECKDRSIAIKRIAQSLIGNYLHVTERIS KSGNPELKRLGLMPGYHQLLKDCEENNNKLSLPMLRKALTQAHSNLSSYVRFIGVQRFAE MVGAPEDAPLFQEALQQGNTIVLTNALVAYLVHGMDNVSRLNSSEKENLIKKYLGTQLSL LYKPLVMEFSGPCAVTREILPLLPTGEPTRYIENLKQPDAQILRVLQTHACVAGKTNFTS DNIPNWITSSEEVERTQSLRTDGLSWMPSEQARLSK |
| EMBL CDS | AAU28909.1 |
| Sequence length | 636 AA |
| Subcellular Location | Host cell plasma membrane |
| Function in Native Organism | 1) It is a secretory glycosyltransferase which glycosylates the elongation factor protein of host. |
| String | 272624.lpg2862. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser) linked |
| CAZY Family | GT88 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-Glc |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Glc) |
| Method of Glycan Indentification | Immunoblot using anti-Lgt serum |
| Experimental_strategies | In vitro and In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | EF1A |
| Litrature | |
| Year Of Validation | 2008 |
| Reference | Belyi, Y., Tabakova, I., Stahl, M., & Aktories, K. (2008). Lgt: a family of cytotoxic glucosyltransferases produced by Legionella pneumophila. Journal of bacteriology, 190(8), 3026-3035. |
| Authors | Belyi, Y., Tabakova, I., Stahl, M., & Aktories, K. |
| Research groups | Gamaleya Research Institute, Moscow 123098, Russia. |
| Corresponding Author | Aktories, K. |
| Contacts | Gamaleya Research Institute, Moscow 123098, Russia. |
| Reference | Hurtado-Guerrero, R., Zusman, T., Pathak, S., Ibrahim, A.F., Shepherd, S., Prescott, A., Segal, G. & Van Aalten, D. M. (2010). Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase. Biochemical Journal, 426(3), 281-292 |
| Authors | Hurtado-Guerrero, R., Zusman, T., Pathak, S., Ibrahim, A.F., Shepherd, S., Prescott, A., Segal, G. & Van Aalten, D. M. |
| Research groups | Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK. |
| Corresponding Author | Van Aalten, D. M. |
| Contacts | Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK. |