ProGT32 (GT)
| ProGT ID | ProGT32 (GT) |
| Organism Information | |
| Organism Name | Peptoclostridium difficile (strain 630) (Clostridium difficile) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Clostridiaceae Order: Clostridiales Class: Clostridia Phylum: Firmicutes |
| Taxonomic ID (NCBI) | 272563 |
| Genome Information | |
| Gene Bank | AM180355 |
| EMBL | AM180355 |
| Gene Information | |
| Gene Name | CD0240 |
| NCBI Gene ID | 4916758 |
| Protein information | |
| Protein Name | Glycosyltransferase |
| UniProtKB/ SwissProt ID | Q18CX6 |
| NCBI Ref Seq | WP_011860691.1 |
| UniProtKB Sequence | >tr|Q18CX6|Q18CX6_PEPD6 Glycosyltransferase OS=Peptoclostridium difficile (strain 630) GN=CD630_02400 PE=4 SV=1 MLLSIVMMVKNEENILEKTLKSLSVLRNSIESELIIVDTGSTDNTIRIAKQYTEKVYFHN WNDDFSSMRNISISYAKGEWLLILDADEVLIDYSNIIKFFESGLNKKFNSASVRLKNLYS FDKKLYGYCSVLRFFKNLDFKYCGKVHEQPMYKNPIFNNIADFEHYGYLFEDEEIRINKV KRNEKLLLEELKEDEKSPYTNYQIGKNLIILGKYQEALDYLEKSYKLYSKLEIVPGYLIT NLAKVYLYLGKAKKCEKLCLKYINKDRNNIDIYYHIAQAQVDLGKYENSIDSYKRYIYLL DNYEMSTQANSLLSDTDAVGFRDEAIITLIKIYYKLEKYDLVISEYDNIEDVEKKKNVYF SLFMSLYKLNRFEDIKNYYREIPNSKVERDSFYKSMEDFINNIKDDEKELIYKSLCDIEG NYGLLNKIRINKHVSLDTCRKILNEEKDIVYASVIKIAFEEKVDLLDIFYNMEYVWIEKY LRYILALERSFNVSIYKYIISCPNTSEIEKIRVYKVISKILLESVNLSDKKYKELFYIYI MYSYKYIKYIYSNLSDCELIKFVCNDIDKFTLEFKHVLDGKRKINNPNEKLEYLHNMRIL LNEYPFYNRIIKIFISELEESITESEEFKLLKDNFIIRIENMIENGKINDAKLLIEDYSK TFADDVKILNIKAILFMCEDKFEEADFALKKALSLDLENEDTIYNIEYLENLK |
| EMBL CDS | CAJ67061.1 |
| Sequence length | 713 AA |
| Function in Native Organism | 1) Inactivation of a glycosyltransferase gene (CD0240) resulted in an inability to produce flagellar filaments at the cell surface. |
| String | 272563.CD0240. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT2 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-HexNAc |
| Glycan Information | |
| Glycan transferred | Monosaccharide (HexNAc) |
| Method of Glycan Indentification | NanoLC-MS/MS, and MS/MS (ETD) |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | FliC |
| ProGPdb ID | ProGP298 |
| Litrature | |
| Year Of Validation | 2009 |
| Reference | Twine, S. M., Reid, C. W., Aubry, A., McMullin, D. R., Fulton, K. M., Austin, J., & Logan, S. M. (2009). Motility and flagellar glycosylation in Clostridium difficile. Journal of bacteriology, 191(22), 7050-7062. |
| Authors | Twine, S. M., Reid, C. W., Aubry, A., McMullin, D. R., Fulton, K. M., Austin, J., & Logan, S. M. |
| Research groups | Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada. |
| Corresponding Author | Logan, S. M. |
| Contacts | Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada. |