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ProGT46 (SunS)

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ProGT ID	ProGT46 (SunS)
ProGT Pathway
Organism Information
Organism Name	Bacillus subtilis 168
Clinical Implication	Pathogenic
Domain	Bacteria
Phylum	Firmicutes
Classification	Family: Bacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)	224308
Genome Information
Gene Bank	AL009126
EMBL	AL009126
Gene Information
Gene Name	sunS
NCBI Gene ID	939123
NCBI Reference Sequence	NC_000964.3.
Protein information
Protein Name	SunS
UniProtKB/ SwissProt ID	O31986
NCBI Ref Seq	NP_390028.1
UniProtKB Sequence	>sp\|O31986\|SUNS_BACSU SPBc2 prophage-derived glycosyltransferase SunS OS=Bacillus subtilis (strain 168) GN=sunS PE=1 SV=1 MKLSDIYLELKKGYADSLLYSDLSLLVNIMEYEKDIDVMSIQSLVAGYEKSDTPTITCGI IVYNESKRIKKCLNSVKDDFNEIIVLDSYSTDDTVDIIKCDFPDVEIKYEKWKNDFSYAR NKIIEYATSEWIYFIDADNLYSKENKGKIAKVARVLEFFSIDCVVSPYIEEYTGHLYSDT RRMFRLNGKVKFHGKVHEEPMNYNHSLPFNFIVNLKVYHNGYNPSENNIKSKTRRNINLT EEMLRLEPENPKWLFFFGRELHLLDKDEEAIDYLKKSINNYKKFNDQRHFIDALVLLCTL LLQRNNYVDLTLYLDILETEYPRCVDVDYFRSAILLVDMQNKLTSLSNMIDEALTDERYS AINTTKDHFKRILISLNIQLENWERVKEISGEIKNDNMKKEIKQYLANSLHNIEHVLKGI EV
EMBL CDS	CAB14063.1
Sequence length	422 AA
Additional Information	1) SunS is the S-glycosyltransferase characterized in vitro which glycosylates SunA peptide with one hexose. 2) SunS recognizes alpha-helix at the N-terminal of the substrate peptide and modifies the Cys residue present in the loop region of SunA. 3) SunS does not modify SunA-C22S mutant, therefore displays high chemoselectivity towards thiol group of a cysteine residue.
Glycosyltransferase Information
Glycosylation Type	S- (Cys) linked
CAZY Family	GT2
EC Number (BRENDA)	2.4.1.-
Mechanism of Glycan Transfer	Sequential
Donor Type	Nucleotide activated suagrs
Donor Specificity	UDP-Glc is the most preferred substrate but it can accept UDP-Gal, UDP-GlcNAc, GDP-Man and UDP-Xyl, though with lesser efficiency.
Glycan Information
Glycan transferred	Monosaccharides (Glc, GlcNAc, Gal, Man and Xyl)
Experimental_strategies	In vitro
Acceptor Subtrate Information
Acceptor Substrate name	SunA
ProGPdb ID	ProGP400
Acceptor Substrate name	SunA-C22S
Acceptor Substrate name	SunAG21A
Acceptor Substrate name	SunAG23A
Acceptor Substrate name	SunAG21K
Acceptor Substrate name	SunAG23K
Acceptor Substrate name	SunAG21F
Acceptor Substrate name	SunAG23F
Acceptor Substrate name	SunAG21E
Acceptor Substrate name	SunAG23E
Acceptor Substrate name	SunA-Xa-T19C
Acceptor Substrate name	SunA-Xa-∆I20
Acceptor Substrate name	SunA-Xa-S16-G17-insAAA
Acceptor Substrate name	SunA-Xa-Q13G-A15G
Litrature
Year Of Validation	2011
Reference	Oman, T. J., Boettcher, J. M., Wang, H., Okalibe, X. N., & Van Der Donk, W. A. (2011). Sublancin is not a lantibiotic but an S-linked glycopeptide. Nature chemical biology, 7(2), 78.
Authors	Oman, T. J., Boettcher, J. M., Wang, H., Okalibe, X. N., & Van Der Donk, W. A.
Research groups	Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Corresponding Author	Van Der Donk, W. A.
Contacts	Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Reference	Wang, H., & Van Der Donk, W. A. (2011). Substrate selectivity of the sublancin S-glycosyltransferase. Journal of the American Chemical Society, 133(41), 16394-16397.
Authors	Wang, H., & Van Der Donk, W. A.
Research groups	Howard Hughes Medical Institute and Roger Adams Laboratory, Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Corresponding Author	Van Der Donk, W. A.
Contacts	Howard Hughes Medical Institute and Roger Adams Laboratory, Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.