ProGT48 (DdPglB)
| ProGT ID | ProGT48 (DdPglB) |
| Organism Information | |
| Organism Name | Desulfovibrio desulfuricans strain G20 |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Desulfovibrionaceae Order: Desulfovibrionales Class: Deltaproteobacteria or Deltabacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 207559 |
| Genome Information | |
| Gene Bank | CP000112 |
| EMBL | CP000112 |
| Gene Information | |
| Gene Name | Dde3699 |
| Protein information | |
| Protein Name | DdPglB |
| UniProtKB/ SwissProt ID | Q30V04 |
| NCBI Ref Seq | ABB40492.1 |
| UniProtKB Sequence | >sp|B9KDD4|PGLB_CAMLR Undecaprenyl-diphosphooligosaccharide--protein glycotransferase OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=pglB PE=1 SV=1 MKLQQNFTDNNSIKYTCILILIAFAFSVLCRLYWVAWASEFYEFFFNDQLMITTNDGYAF AEGARDMIAGFHQPNDLSYFGSSLSTLTYWLYSILPFSFESIILYMSAFFASLIVVPIIL IAREYKLTTYGFIAALLGSIANSYYNRTMSGYYDTDMLVLVLPMLILLTFIRLTINKDIF TLLLSPVFIMIYLWWYPSSYSLNFAMIGLFGLYTLVFHRKEKIFYLTIALMIIALSMLAW QYKLALIVLLFAIFAFKEEKINFYMIWALIFISILILHLSGGLDPVLYQLKFYVFKASDV QNLKDAAFMYFNVNETIMEVNTIDPEVFMQRISSSVLVFILSFIGFILLCKDHKSMLLAL PMLALGFMALRAGLRFTIYAVPVMALGFGYFLYAFFNFLEKKQIKLSLRNKNILLILIAF FSISPALMHIYYYKSSTVFTSYEASILNDLKNKAQREDYVVAWWDYGYPIRYYSDVKTLI DGGKHLGKDNFFSSFVLSKEQIPAANMARLSVEYTEKSFKENYPDVLKAMVKDYNKTSAK DFLESLNDKDFKFDTNKTRDVYIYMPYRMLRIMPVVAQFANTNPDNGEQEKSLFFSQANA IAQDKTTGSVMLDNGVEIINDFRALKVEGASIPLKAFVDIESITNGKFYYNEIDSKAQIY LLFLREYKSFVILDESLYNSSYIQMFLLNQYDQDLFEQITNDTRAKIYRLKR |
| EMBL CDS | ABB40492.1 |
| Sequence length | 722 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) Transfer the C.jejuni heptasaccharide to the Acceptor protein AcrA in E.coli. |
| Additional Information | 1) DdPglB does not follow -2 rule, negatively charged residue at the ?2 position of the glycosylation sequon N-X-S/T. 2) PglBDd has relaxed glycan specificity, It can transfer monosaccharide and polysaccharide to AcrA in E. coli apart from C. jejuni heptasaccharide. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.19.13108 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | Lipid linked sugars |
| Donor Specificity | UndPP-Heptasaccharide |
| Glycan Information | |
| Glycan transferred | Heptasaccharide (GalNAc- 1,4-GalNAc- 1,4-(Glc 1,3)-Gal-NAc- 1,4- GalNAc- 1,4-GalNAc- 1,3-Bac), where Bac is bacillosamine(2,4-diacetamido-2,4,6-trideoxyglucose). |
| Method of Glycan Indentification | LC-MS, and MALDI-TOF/TOF |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | AcrA |
| ProGPdb ID | ProGP217 |
| Acceptor Substrate name | scFv13-R4 AQNAT |
| Acceptor Substrate name | scFv13-R4 DQNAT |
| Acceptor Substrate name | scFv13-R4 QQNAT |
| Acceptor Substrate name | scFv13-R4 CQNAT |
| Acceptor Substrate name | scFv13-R4 EQNAT |
| Acceptor Substrate name | scFv13-R4 FQNAT |
| Acceptor Substrate name | scFv13-R4 GQNAT |
| Acceptor Substrate name | scFv13-R4 HQNAT |
| Acceptor Substrate name | scFv13-R4 LQNAT |
| Acceptor Substrate name | scFv13-R4 MQNAT |
| Acceptor Substrate name | scFv13-R4 NQNAT |
| Acceptor Substrate name | scFv13-R4 PQNAT |
| Acceptor Substrate name | scFv13-R4 SQNAT |
| Acceptor Substrate name | scFv13-R4 TQNAT |
| Acceptor Substrate name | scFv13-R4 YQNAT |
| Litrature | |
| Year Of Validation | 2011 |
| Reference | Ielmini, M. V., & Feldman, M. F. (2010). Desulfovibrio desulfuricans PglB homolog possesses oligosaccharyltransferase activity with relaxed glycan specificity and distinct protein acceptor sequence requirements. Glycobiology, 21(6), 734-742. |
| Authors | Ielmini, M. V., & Feldman, M. F. |
| Research groups | Alberta Ingenuity Centre for Carbohydrate Science, Department of Biological Sciences, University of Alberta, Edmonton, Alta, Canada |
| Corresponding Author | Feldman, M. F. |
| Contacts | Alberta Ingenuity Centre for Carbohydrate Science, Department of Biological Sciences, University of Alberta, Edmonton, Alta, Canada |
| Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. (2015). Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5, 15237. |
| Authors | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. |
| Research groups | 1 School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853 USA.
2 Department of Microbiology, Cornell University, Ithaca, NY 14853 USA.
3 Comparative Biomedical Sciences, Cornell University, Ithaca, NY 14853 USA.
4 Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York. |
| Corresponding Author | DeLisa, M. P. |
| Contacts | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |