ProGT49 (PglA)
| ProGT ID | ProGT49 (PglA) |
| Organism Information | |
| Organism Name | Francisella tularensis SCHU S4 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Francisellaceae Order: Thiotrichales Class: Gammaproteobacteria Phylum: Proteobacteria |
| Taxonomic ID (NCBI) | 177416 |
| Genome Information | |
| Gene Bank | AJ749949 |
| EMBL | CP010290 |
| Gene Information | |
| Gene Name | FTT_0905 |
| NCBI Gene ID | 3191692 |
| NCBI Reference Sequence | AJ749949.2 |
| Protein information | |
| Protein Name | PglA |
| UniProtKB/ SwissProt ID | Q5NGE0 |
| NCBI Ref Seq | YP_169902.1 |
| UniProtKB Sequence | >tr|Q5NGE0|Q5NGE0_FRATT Type IV pili glycosylation protein OS=Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) GN=FTT_0905 PE=4 SV=1 MIEVINYLKKISYADYIFYFLTLAIFFVFLMPFMHYIVLNLIKVTSGSTVSYFNDTLAAN IYLFIDRYYIFYTVLGVASVAIFLNKRIFSEVIELFSSFNLSIRYAIALFFIFGVISSVF AISPSIAFKGVSVTFLQFFCILFIAVYIRDNSKAVRFFYTIILLSLIFFGGSLFLQLSLA NYSVYGSYIAGNIQKVLLYMYNCLNPRFLDNYFSWFMPLILLPWFVDLRPIYKLGSFIAL ILAWFILINHGFRTIFAEYIIILPLLYIFARRYFKIAFVVLVLALVYAGLLELFYNYFIA VVDHSNVKSSADLVRGGFSGRIPLWKEAFWVGVEHPLTGVGQWNYLAITKRPDGYPHNLL LEIWSQWGIPAFIAAAVVIITTVRNLYKKRLEICLNSYQCIFVMMLTAGMIDGMLNAMFK TSLGLFGCVFVFGFCLSIFVKQPQQDTSVSATSYAIVGLSIFASIFCIAILPLIFPPLWI |
| EMBL CDS | AJI68644.1 |
| Sequence length | 480 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) Transfer the pentasaccharide into the native substrate PilA. |
| String | 177416.FTT_0905 |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Donor Type | Lipid linked sugars |
| Donor Specificity | Lipid linked glycan |
| Glycan Information | |
| Glycan transferred | Pentasaccharide (HexNac-Hex-Hex-HexNac-HexNac) |
| Method of Glycan Indentification | MS/MS (CID) , MS ETD |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | PliA |
| ProGPdb ID | ProGP250 |
| Litrature | |
| Year Of Validation | 2011 |
| Reference | Egge-Jacobsen, W., Salomonsson, E.N., Aas, F.E., Forslund, A.L., Winther-Larsen, H.C., Maier, J., Macellaro, A., Kuoppa, K., Oyston, P.C.F., Titball, R.W. & Thomas, R. M. (2011). O-linked glycosylation of the PilA pilin protein of Francisella tularensis: identification of the endogenous protein-targeting oligosaccharyltransferase and characterization of the native oligosaccharide. Journal of bacteriology, JB-00383. |
| Authors | Egge-Jacobsen, W., Salomonsson, E.N., Aas, F.E., Forslund, A.L., Winther-Larsen, H.C., Maier, J., Macellaro, A., Kuoppa, K., Oyston, P.C.F., Titball, R.W. & Thomas, R. M. |
| Research groups | Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway |
| Corresponding Author | Thomas, R. M. |
| Contacts | Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway |