ProGT51 (AglB)
| ProGT ID | ProGT51 (AglB) |
| Organism Information | |
| Organism Name | Sulfolobus acidocaldarius |
| Clinical Implication | Non-pathogenic |
| Domain | Archaebacteria |
| Phylum | Crenarchaeota |
| Classification | Family: Sulfolobaceae Order: Sulfolobales Class: Thermoprotei or Crenarchaeota Division or phylum: "Crenarchaeota" |
| Taxonomic ID (NCBI) | 330779 |
| Genome Information | |
| Gene Bank | CP000077 |
| EMBL | CP000077 |
| Gene Information | |
| Gene Name | saci1274 |
| NCBI Gene ID | 3473075 |
| NCBI Reference Sequence | NC_007181.1. |
| Protein information | |
| Protein Name | AglB |
| UniProtKB/ SwissProt ID | Q4J9B4 |
| NCBI Ref Seq | WP_011278118.1 |
| UniProtKB Sequence | >tr|Q4J9B4|Q4J9B4_SULAC Conserved membrane protein OS=Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) GN=Saci_1274 PE=4 SV=1 MQSTSILARIDKFKFLDAIIIGSLALFSILIRIISITAFPQTINGFDSWYLFYNALLIVK AGGNWYAVPPDVHAWFPWGYFIELENTIGLPFLVALFSVPFYSIFGQNIVYTLTLVSPIV LDGIGVVAAFLAVESITNSRVGGYIAAAITAFTPSLTYKNILGSLPKTSWGGVFVLFTIY FLSLAIKKKKPLYGIPAGIMIFLANITWGGYTYIDISLAIAAFLIVLFNKNDEISAKTLT ISGITAAFLTSLSPNTIGFMSEVAHGLALLIIPLFLYLDLYLRRVLPKDIVDSKNIVIGA AIILLVSLVVLGSVAFKVQLIPSRYYAIINPFFQFTVPIDRTVAEYIPQSIAAMIQDFGI GLFLSIIGIYFLLTRKQDMAGIWLVVLGAASIYGTSEQPYLFNYTIYIVAALAGVAVAEL FSRFMERKIRIAPILMLTLIGVALLADAGIAVEASYAPQALINSSTSYLTTNYAWISALD WINQNTPNNAFILSWWDYGYWIHAVGNRTVIDENNTLNGTQIKLMAEMFLNNESFAVNVL ENDFHLYPYGNPNYTRPVYIVAYDAVTEYIVNNQYPVWFIGYPTNFPGTFIGYTTSLGDI AKAIGAMTTIAGYNTNSYVNTTYINETASYAAQYNSQLASIIANSLPMAWTPKTYNSLIG SMFIEAIQSLNQGPVQAPFSISLSQLLQSSSTSLYNPNALLPRVNLMYFKPVYIALFPLS VTNALGGEAIVYIMVYIYQFVMPNVIIPPTISTA |
| EMBL CDS | AAY80616.1 |
| Sequence length | 754 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) The composition of SlaA glycan is changed in the ?agl3 deletion mutant, this significantly reduces the growth rate at elevated salt concentrations. |
| String | 330779.Saci_1274 |
| Additional Information | 1) AglB catalyzes the final step of the transfer of glycan to S-layer protein as well as to other surface exposed proteins. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 6160 |
| Mechanism of Glycan Transfer | En bloc |
| Accessory GT ID | ProGT51.1ProGT51.2ProGT51.3 |
| Glycan Information | |
| Glycan transferred | Hexasaccharide (Glc1Man2GlcNAc2QuiS) |
| Method of Glycan Indentification | MALDI-MS, NMR, and nanoLC ES-MS/MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Cytochrome b558/566 subunit A |
| ProGPdb ID | ProGP150 |
| Acceptor Substrate name | SlaA (S- layer protein) |
| ProGPdb ID | ProGP344 |
| Acceptor Substrate name | FlaB |
| Litrature | |
| Year Of Validation | 2011 |
| Reference | Meyer, B.H., Zolghadr, B., Peyfoon, E., Pabst, M., Panico, M., Morris, H.R., Haslam, S.M., Messner, P., Schäffer, C., Dell, A. & Albers, S.V., (2011). Sulfoquinovose synthase–an important enzyme in the N?glycosylation pathway of Sulfolobus acidocaldarius. Molecular microbiology, 82(5), pp.1150-1163. |
| Authors | Meyer, B.H., Zolghadr, B., Peyfoon, E., Pabst, M., Panico, M., Morris, H.R., Haslam, S.M., Messner, P., Schäffer, C., Dell, A. & Albers, S.V., |
| Research groups | Molecular Biology of Archaea, Max-Planck Institute for terrestrial Microbiology, Karl-von-Frisch-Strasse 10, 35043 Marburg. |
| Corresponding Author | Albers, S.V., |
| Contacts | Molecular Biology of Archaea, Max-Planck Institute for Terrestrial Microbiology, Marburg, Germany. |
| Reference | Meyer, B. H., Peyfoon, E., Dietrich, C., Hitchen, P., Dell, A., & Albers, S. V. (2013). Agl16, a thermophilic glycosyltransferase, mediating the last step of the N-glycan biosynthesis in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. Journal of bacteriology, JB-00035. |
| Authors | Meyer, B. H., Peyfoon, E., Dietrich, C., Hitchen, P., Dell, A., & Albers, S. V. |
| Research groups | Molecular Biology of Archaea, Max-Planck Institute for Terrestrial Microbiology, Marburg, Germany. |
| Corresponding Author | Albers, S. V. |
| Contacts | Molecular Biology of Archaea, Max-Planck Institute for Terrestrial Microbiology, Marburg, Germany. |
| Reference | Meyer, B. H., & Albers, S. V. (2014). AglB, catalyzing the oligosaccharyl transferase step of the archaeal N?glycosylation process, is essential in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. MicrobiologyOpen, 3(4), 531-543. |
| Authors | Meyer, B. H., & Albers, S. V. |
| Research groups | Molecular Biology of Archaea, Max-Planck Institute for terrestrial Microbiology, Karl-von-Frisch-Strasse 10, 35043, Marburg. |
| Corresponding Author | Albers, S. V. |
| Contacts | Molecular Biology of Archaea, Max-Planck Institute for terrestrial Microbiology, Karl-von-Frisch-Strasse 10, 35043, Marburg. |
| Reference | Meyer, B. H., Shams-Eldin, H., & Albers, S. V. (2017). AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate: dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7. Extremophiles, 21(1), 121-134. |
| Authors | Meyer, B. H., Shams-Eldin, H., & Albers, S. V. |
| Research groups | 1 Molecular Biology of Archaea, Institute of Biology, University of Freiburg, Schaenzlestrasse 1, 79211, Freiburg, Germany.
2 Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
3 Institute of Virology, Hans-Meerwein-Str. 2, 35043, Marburg, Germany.
4 Molecular Biology of Archaea, Institute of Biology, University of Freiburg, Schaenzlestrasse 1, 79211, Freiburg, Germany. |
| Corresponding Author | Albers, S. V. |
| Contacts | Molecular Biology of Archaea, Institute of Biology, University of Freiburg, Schaenzlestrasse 1, 79211, Freiburg, Germany. |