ProGT62 (PimE)
| ProGT ID | ProGT62 (PimE) |
| Organism Information | |
| Organism Name | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) |
| Clinical Implication | Non-pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Actinobacteria Class : Actinomycetia Orders : Corynebacteriales Family : Mycobacteriaceae Genus : Mycobacterium Species : smegmatis Strain : ATCC 700084 / mc(2)155 |
| Taxonomic ID (NCBI) | 246196 |
| Genome Information | |
| Gene Bank | CP000480.1 |
| EMBL | CP000480 |
| Gene Information | |
| Gene Name | Msmeg_5149 |
| NCBI Reference Sequence | NC_008596.1. |
| Protein information | |
| Protein Name | PimE |
| UniProtKB/ SwissProt ID | A0R2K8 |
| NCBI Ref Seq | YP_889686.1 |
| UniProtKB Sequence | >sp|A0R2K8|PIME_MYCS2 Polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol pentamannoside mannosyltransferase OS=Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) GN=pimE PE=1 SV=2 MRVNGYRGAKVGAVDTTSPPEVPASARLQRLAPMLLVVSILARLAWTYLVPNGANFVDLH VYVGGADALDGPGALYDYVYADQTPDFPLPFTYPPFAAIVFYPLHLLPFGVVAFIWQIGI IAALYGVVRVSQRLMGLQSQRRVAMLWTALGIWTEPLRSTFDYGQVNVVLVLAVLCAVST TRWWLSGLLVGLAAGIKLTPAVAGLYFLGARRWAAVACSAAVFFATVGVSWLVVGAQARR YFTELLGDADRIGPIGTSFNQSWRGGISRILGHDAGFGPLVLIGIGITAVLALLAWRAIG GAQDRLGGILVVSLFGLVLSPISWTHHWVWLIPLMMWLLHGPLSALRGARILGWGWLALT LLGVPWLLSFAQPTIWEIGRPWYLAWAGLVYIVATLATLGWIAFSRKGSG |
| EMBL CDS | ABK75499.1 |
| Sequence length | 410 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) Inactivation of the M. tuberculosis Rv1002c gene impairs Apa protein O-mannosylation and the diminished ability of Mtb mutants to form colonies on solid medium. |
| String | 246196.MSMEG_5149 |
| Additional Information | 1) Mutation in Rv1002c gene showed the essential role of O-mannosylation in virulence and growth. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT87 |
| EC Number (BRENDA) | 2.4.1.- |
| Glycan Information | |
| Glycan transferred | Mannosyl chain elongation through alpha1-2 linkages |
| Method of Glycan Indentification | Nano-LC-MS/MS and (ETD) MS/MS |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | ABC-type amino acid transport system secreted component |
| ProGPdb ID | ProGP459 |
| Acceptor Substrate name | Putative protein |
| ProGPdb ID | ProGP460 |
| Acceptor Substrate name | Putative uncharacterized protein |
| ProGPdb ID | ProGP461 |
| Acceptor Substrate name | Putative uncharacterized protein |
| ProGPdb ID | ProGP462 |
| Acceptor Substrate name | Immunogenic protein MPT63 |
| ProGPdb ID | ProGP467 |
| Acceptor Substrate name | Putative protein |
| ProGPdb ID | ProGP464 |
| Acceptor Substrate name | Immunogenic protein MPT63 |
| ProGPdb ID | ProGP461 |
| Acceptor Substrate name | Putative protein |
| ProGPdb ID | ProGP466 |
| Acceptor Substrate name | Alanine and proline-rich secreted protein apa |
| ProGPdb ID | ProGP467 |
| Acceptor Substrate name | FasC (fasciclin domain protein) |
| ProGPdb ID | ProGP435 |
| Litrature | |
| Year Of Validation | 2013 |
| Reference | Liu, C.F., Tonini, L., Malaga, W., Beau, M., Stella, A., Bouyssié, D., Jackson, M.C., Nigou, J., Puzo, G., Guilhot, C. and Burlet-Schiltz, O., 2013. Bacterial protein-O-mannosylating enzyme is crucial for virulence of Mycobacterium tuberculosis. Proceedings of the National Academy of Sciences, 110(16), pp.6560-6565. |
| Corresponding Author | National Center for Scientific Research, Institute of Pharmacology and Structural Biology, F-31077 Toulouse, France. |