ProGT63 (NleB1)
| ProGT ID | ProGT63 (NleB1) |
| Organism Information | |
| Organism Name | Escherichia coli EPEC |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Enterobacterales Family : Enteerobacteriaceae Genus : Escherichia Species : coli Strain : EPEC |
| Taxonomic ID (NCBI) | 574521 |
| Genome Information | |
| Gene Bank | FM180568.1 |
| EMBL | FM180568 |
| Gene Information | |
| Gene Name | nleB1 |
| NCBI Reference Sequence | FM180568 |
| Protein information | |
| Protein Name | NleB1 |
| UniProtKB/ SwissProt ID | B7UI21 |
| NCBI Ref Seq | WP_012578998.1? |
| UniProtKB Sequence | >tr|B7UI21|B7UI21_ECO27 T3SS secreted effector NleB homolog OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=E2348C_3231 PE=4 SV=1 MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYSLEPIDERTPILFQWFEARPERYEKG EVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKKEFLNLENEHQNIKIIYRA DVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLREELKNIPEGKDSLIESYAEKR EHTWFDFFRNLAILKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIA VHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSL HNYNAFCDFIEFKHENIIPNTSMYTSSSW |
| EMBL CDS | CAS10779.1 |
| Sequence length | 329 AA |
| Subcellular Location | Host cell plasma membrane |
| Additional Information | 1) NleB, N-acetylglucosamine (GlcNAc) transferase modify a conserved arginine in death domains proteins of the host such as TRADD and FADD.
2) NleB GlcNAcylation of death domains blocked homotypic/heterotypic death domain interactions and assembly of the oligomeric TNFR1 complex which results in disrupting TNF signaling in EPEC-infected cells, including NFkB signaling, apoptosis, and necroptosis. 3) The arginine GlcNAc transferase activity of NleB is required for bacterial colonization in the mouse model of EPEC infection. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Arg) linked |
| CAZY Family | GTNC |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Method of Glycan Indentification | LC-MS, MALDI-TOF, MS (ETD) |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | TRADD |
| Acceptor Substrate name | FADD |
| Acceptor Substrate name | SdrE |
| Litrature | |
| Year Of Validation | 2013 |
| Reference | Li, S., Zhang, L., Yao, Q., Li, L., Dong, N., Rong, J., Gao, W., Ding, X., Sun, L., Chen, X. and Chen, S., 2013. Pathogen blocks host death receptor signalling by arginine GlcNAcylation of death domains. Nature, 501(7466), pp.242-246. |
| Corresponding Author | College of Biological Sciences, China Agricultural University, Beijing 100094, China |
| Reference | Pearson, J.S., Giogha, C., Ong, S.Y., Kennedy, C.L., Kelly, M., Robinson, K.S., Lung, T.W.F., Mansell, A., Riedmaier, P., Oates, C.V. and Zaid, A., 2013. A type III effector antagonizes death receptor signalling during bacterial gut infection. Nature, 501(7466), pp.247-251. |
| Corresponding Author | Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia |