ProGT67 (SdgB)
| ProGT ID | ProGT67 (SdgB) |
| Organism Information | |
| Organism Name | Staphylococcus aureus |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Clostridiaceae Order: Clostridiales Class: Clostridia Phylum: Firmicutes |
| Taxonomic ID (NCBI) | 367830 |
| Genome Information | |
| Gene Bank | CP000255 |
| EMBL | CP000255 |
| Gene Information | |
| Gene Name | SAUSA300_0550 |
| NCBI Reference Sequence | ABD21647.1 |
| Protein information | |
| Protein Name | SdgB |
| UniProtKB/ SwissProt ID | A0A0H2XGN0 |
| NCBI Ref Seq | WP_001106113.1 |
| UniProtKB Sequence | >tr|A0A0H2XGN0|A0A0H2XGN0_STAA3 Glycosyl transferase, group 1 family protein OS=Staphylococcus aureus (strain USA300) GN=SAUSA300_0550 PE=4 SV=1 MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRYLYRNAQNYITSSDYIN MYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPKYR ILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYFSYKD GKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNLTPESIP VVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKRINHTIPVV NIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYVPKIELHMYGF GSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITSNMEGFSLALLESLAHGV PVISYDIKYGPNELITSDFNGYLITKNDEDALFDKVKYVIDHPEVQQRLSKGSLAKAQQY SKASLIKQWDQFVRLI |
| EMBL CDS | ABD21647.1 |
| Sequence length | 496 AA |
| Function in Native Organism | 1) SdgB transfer the first GlcNAc to the SDR Protein. |
| Potential Application | |
| Additional Information | 1) SdgB adds GlcNAc residue to the SDR protein. 2) SdgB creates an immunodominant epitope for highly opsonic human antibodies by transferring GlcNAc residue on SDR-proteins. 3) aggB (sdgA) and aggC (sdgB) are involved in GlcNAc modification of the ClfA SD repeats. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT4 |
| EC Number (BRENDA) | 2.4.1.109 |
| Mechanism of Glycan Transfer | Sequential |
| Acceptor specificity Sequon_1 | Serine-Aspartate dipeptide-Repeats (SDR) |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-GlcNAc |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Method of Glycan Indentification | LC-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Pls (Plasmin sensitive surface protein) |
| ProGPdb ID | ProGP551 |
| Acceptor Substrate name | ClfASD5 |
| Litrature | |
| Year Of Validation | 2013 |
| Reference | Hazenbos, W.L., Kajihara, K.K., Vandlen, R., Morisaki, J.H., Lehar, S.M., Kwakkenbos, M.J., Beaumont, T., Bakker, A.Q., Phung, Q., Swem, L.R. & Ramakrishnan, S. (2013). Novel staphylococcal glycosyltransferases SdgA and SdgB mediate immunogenicity and protection of virulence-associated cell wall proteins. PLoS pathogens, 9(10), e1003653. |
| Authors | Hazenbos, W.L., Kajihara, K.K., Vandlen, R., Morisaki, J.H., Lehar, S.M., Kwakkenbos, M.J., Beaumont, T., Bakker, A.Q., Phung, Q., Swem, L.R. & Ramakrishnan, S. |
| Research groups | Dept. of Infectious Diseases, Genentech, Inc., San Francisco, California, United States of America. |
| Corresponding Author | Ramakrishnan, S. |
| Contacts | Dept. of Infectious Diseases, Genentech, Inc., San Francisco, California, United States of America |
| Reference | Thomer, L., Becker, S., Emolo, C., Quach, A., Kim, H.K., Rauch, S., Anderson, M., LeBlanc, J.F., Schneewind, O., Faull, K.F. & Missiakas, D. (2013). N-acetylglucosaminylation of serine-aspartate repeat proteins promotes Staphylococcus aureus blood stream infection. Journal of Biological Chemistry, jbc-M113. |
| Authors | Thomer, L., Becker, S., Emolo, C., Quach, A., Kim, H.K., Rauch, S., Anderson, M., LeBlanc, J.F., Schneewind, O., Faull, K.F. & Missiakas, D. |
| Research groups | Department of Microbiology, The University of Chicago, Chicago, Illinois 60637 |
| Corresponding Author | Missiakas, D. |
| Contacts | Department of Microbiology, The University of Chicago, Chicago, Illinois 60637 |