ProGT70 (GtfB)
| ProGT ID | ProGT70 (GtfB) |
| Organism Information | |
| Organism Name | Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) |
| Clinical Implication | Non-pathogenic |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Lactobacillaceae Order: Lactobacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 220668 |
| Genome Information | |
| Gene Bank | AL935263 |
| EMBL | AL935263 |
| Gene Information | |
| Gene Name | tagE6 (lp_2844) |
| NCBI Gene ID | 1063214 |
| Protein information | |
| Protein Name | GtfB |
| UniProtKB/ SwissProt ID | F9URU7 |
| NCBI Ref Seq | WP_003642121.1 |
| UniProtKB Sequence | >tr|F9URU7|F9URU7_LACPL Poly(Glycerol-phosphate) alpha-glucosyltransferase OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) GN=tagE6 PE=4 SV=1 MYYFVNTSINPKKSGIEHAEMKRLALFNQHHVPAKMVTRFFSLSLHQTLTAAGIAAENQI NLFDFIQHSSERPTTKMTIEDLHLAPDLRVQSVGRHYEVYQNDQLLMKVNTFLNTQKQLN NVQYYDQNGKLVKTDWYDTRGFKGLEQFYAADGQVASEQVFNPDGEVVYQTFHMADRQNQ VQNSLYRFINYEGQDWSFAGETAMTTFFLDELNRRTPHAVFVIDRTYELAYAALHMRTKA FKVMHLHSNHVNDPAHPKTASLNFNYAYALNNLDQWNGVIASTKQQTADFQARFGNQPAV YTIPVGIVDDATLNAPKPSFDQRTAGKIIFVARLAEEKQQDQVIRAFKKVLAQVPNATLD FWGYANGDTGKQLHQLVEQLGLKAAVTFNDYTTDLTAVYNDAQLAMLTSRAEGFALALLE GQAHGVPQIAYDVKYGPRDIIRDGHDGQLVPVNDENALATAMIDLLTQPKKLAMYSQQAY QDAQRFSTTAVWQQWQPLMKAAADFYQVEGATQR |
| EMBL CDS | CCC79936.1 |
| Sequence length | 504 AA |
| Function in Native Organism | 1) Deletion studies of Six TagE show that both Both TagE5 and TagE6 are required for protein glycosylation in L. plantarum WCFS1. |
| String | 220668.lp_2844 |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT4 |
| EC Number (BRENDA) | 2.4.1.119 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-GlcNAc |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Method of Glycan Indentification | MALDI-TOF MS/MS and LC-MS/MS |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Acm2 |
| ProGPdb ID | ProGP436 |
| Litrature | |
| Year Of Validation | 2014 |
| Reference | Lee, I.C., van Swam, I.I., Tomita, S., Morsomme, P., Rolain, T., Hols, P., Kleerebezem, M. & Bron, P. A. (2014). GtfA and GtfB are both required for protein O-glycosylation in Lactobacillus plantarum. Journal of bacteriology, JB-01401. |
| Authors | Lee, I.C., van Swam, I.I., Tomita, S., Morsomme, P., Rolain, T., Hols, P., Kleerebezem, M. & Bron, P. A. |
| Research groups | TI Food and Nutrition, Wageningen, the Netherlands. |
| Corresponding Author | Bron, P. A. |
| Contacts | TI Food and Nutrition, Wageningen, the Netherlands. |