ProGT73 (STT3)

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ProGT ID ProGT73 (STT3)
Organism Information
Organism NameHaloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei)
Clinical ImplicationNon-pathogenic
DomainArchaebacteria
Classification Phylum : Euryarchaeota
Class : Halobaccteria
Orders : Haloferacales
Family : Helicobacteraceae
Genus : Haloferax
Species : mediterranei
Strain : TCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4
Taxonomic ID (NCBI)523841
Genome Information
Gene BankCP001868.2
EMBLCP001868
Gene Information
Gene Namestt3
NCBI Gene ID13027753
NCBI Reference SequenceCP001868 
Protein information
Protein NameSTT3  
UniProtKB/ SwissProt IDI3R4Y8
NCBI Ref SeqWP_004056863.1
UniProtKB Sequence>tr|I3R4Y8|I3R4Y8_HALMT Dolichyl-diphosphooligosaccharide--protein glycosyltransferase OS=Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) OX=523841 GN=stt3 PE=4 SV=1 MSADTERIEDSGSKSVLELAKDWYHVPALLVILVGMLTLRLRSYSNFIRGGEVLFSGNDA WYHLREVTYTVQNWPSTMPFDPWTYFPFGTSVGQFGTIYDQIIATAALVVGLGSPSPDLV AKTLLVAPAVFGTLVAIPTFLIGRRLGGRTGGLFGVLILALLPGTFLRRGLVGFADHNIA EPFFQGFAVVTVMIALTVANREKPVWELVAARDFDALRSTLIWSALAGVAMAIYMWSWPP GVLLVGIFGLFLVLQMVADYVRGRSPEHVAFVGAVSLGVTGLLMFVPLHEMSFSATSFSL LQPVVSFVIALGAVFLAALARFWENNDVDDRGYPVAVGGLAVAGIVLISVVLPDAFNSIA RNFLRTVGFSAGAATRTIGEAQPFLSSSTLQRTGMTAAERIMSEYGFTFFTGLVGAIWLV GKPLVSEGDTRKVGYVFGSLAVIGLIFLAPAIPAGIGGLVGIDSALISLAIVTAIIVGAV LQADYESEHLFVLVWAAIITSAAFTQVRFNYYLAVVVAVMNAYLLREILSFDVIGLADVD RLENVSLGTVAVVAVALLLILTPVLMVPIQLGNAGTSSNALESARSGPGGVTQWQSTFDW MQNNTPEEGEFGGASNEMEYYGTYKATNDFEYADGTYGVMSWWDYGHWITVLGERIPNAN PFQQGAKEAANFLLAPNETQASNVLAEQSEEGNETRYVMVDWQMASTDSKFGAPTIFYNK QNVSSSDFYSAYYGLQRQDGQERLVSSTTLKDQRYYESLMVRLYEYHGSARNPSPIVVDW EERTSRDGSTTFKVTPSDNKPVKSFRTMDAAKAYVANDTTSQIGGLGAFPEERVAALEHY RLVKSSNSTALRSQSYYNSIASAGRTYGIQPRALLGNNPAWVKTFERVPGATVEGDGAPA NATVTARVEMRDPVSGTTFNYTQQAQTDANGEFTMTLPYSTTGYDDYGPDNGYTNVSVRA VDDYTFTGPTSFEGNNTFVTYQATNVSVEEGLVNGAEDGTVQVSLEQTEQEITLNESSSD DSSSDDAETNESTSASIDAGSVPAVAA
EMBL CDSAFK19298.1.
Sequence length1047 AA
Subcellular LocationMembrane (Integral component of membrane)
Additional Information1) AglB of Hfx. mediterranei is able to complement Hfx. volcanii AglB in AglB deleted Hfx. volcanii cell.
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
CAZY FamilyGT66
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferEn bloc
Acceptor specificity Sequon_1Asn-Xaa-Ser/Thr
Donor SpecificityLipid linked sugars
Glycan Information
Glycan transferredHex-(HexA)2-HexA(methyl ester)-Hex 
Method of Glycan IndentificationLC-ESI MS/MS
Acceptor Subtrate Information
Acceptor Substrate name S-layer glycoprotein
ProGPdb ID ProGP71
Litrature
Year Of Validation2014 
Reference Cohen-Rosenzweig, C., Guan, Z., Shaanan, B. and Eichler, J., 2014. Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans. Applied and environmental microbiology, 80(2), pp.486-496.

Corresponding AuthorDepartment of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel.