ProGT77 (GtfA)
| ProGT ID | ProGT77 (GtfA) |
| Organism Information | |
| Organism Name | Staphylococcus aureus |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Clostridiaceae Order: Clostridiales Class: Clostridia Phylum: Firmicutes |
| Taxonomic ID (NCBI) | 450394 |
| Genome Information | |
| Gene Bank | AASB02000025 |
| EMBL | AASB02000025 |
| Gene Information | |
| Gene Name | gtfA |
| NCBI Reference Sequence | AASB02000025? |
| Protein information | |
| Protein Name | GtfA |
| UniProtKB/ SwissProt ID | A0A0E1VPL4 |
| NCBI Ref Seq | WP_000158427.1 |
| UniProtKB Sequence | >tr|A0A0E1VPL4|A0A0E1VPL4_STAA3 Glycosyltransferase Gtf1 OS=Staphylococcus aureus subsp. aureus USA300_TCH959 GN=gtfA PE=3 SV=1 MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQSENIQTLTQNIGFKDQ EVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIVRLYFNGKSSFVTCYLQNE QKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAKLYMRQFYNEDGSIAYNEYID GDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILDRASDIGQAVLQHKGDSKVGVVI HADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFITATDIQNHMVCRQFEQYQGYRPRVY TIPVGSIDALSYPTLSRKPYAMISASRLANEKHIDWLVKAVIVAKRQVPELTFDIYGEGS EKTRLRKIIDTHRAQDYIRLLGHVKLDEIYNDYELFLSASTSEGFGLTLMEAVGSGLGMI GLNVNYGNPTFIRDGENGYLVPFDTDEDSVDDVVAKLAHAIVMYFNNGPQTPHDISYEVA QQFMTQDIILKWETLVQEVLHD |
| EMBL CDS | EES94654.1 |
| Sequence length | 502 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Additional Information | 1) The deletion studies of GtfA and GtfB show that both GtfA-GtfB is involved in the transfer of GlcNAc residues to the peptide backbone of SraP. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT4 |
| EC Number (BRENDA) | 2.4.1.119 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-GlcNAc |
| Glycan Information | |
| Glycan transferred | Oligosaccharides |
| Method of Glycan Indentification | sWGA blotting |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | SraP |
| ProGPdb ID | ProGP245 |
| Litrature | |
| Year Of Validation | 2014 |
| Reference | Li, Y., Huang, X., Li, J., Zeng, J., Zhu, F., Fan, W., & Hu, L. (2014). Both GtfA and GtfB are required for SraP glycosylation in Staphylococcus aureus. Current microbiology, 69(2), 121-126. |
| Authors | Li, Y., Huang, X., Li, J., Zeng, J., Zhu, F., Fan, W., & Hu, L. |
| Research groups | 1 Department of Laboratory Medicine, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina 2 Department of Otorhinolaryngology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina 3. Department of Pediatric Dentistry, Schools of Dentistry and MedicineUniversity of Alabama at BirminghamBirminghamUSA 4. Department of Laboratory MedicineJingzhou First People’s HospitalJingzhouChina |
| Corresponding Author | Hu, L. |
| Contacts | Department of Laboratory Medicine, Union Hospital, Tongji Medical College Huazhong, University of Science and Technology, Wuhan, China. |