ProGT85 (TfpOM2)
| ProGT ID | ProGT85 (TfpOM2) |
| Organism Information | |
| Organism Name | Acinetobacter nosocomialis strain M2 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Moraxellaceae Order: Pseudomonadales Class: Gammaproteobacteria Phylum: Proteobacteria |
| Taxonomic ID (NCBI) | 1217985 |
| Genome Information | |
| Gene Bank | APPP01000010 |
| EMBL | APPP01000010 |
| Gene Information | |
| Gene Name | tfpO |
| Protein information | |
| Protein Name | TfpOM2 |
| Subcellular Location | Membrane (Integral component of membrane) |
| Additional Information | 1) TfpOM2 is required for the glycosylation of pilA at its terminal serine residue. 2) The carboxy-terminal serine 136 of PilAM2 is modified by glycosyltransferase TfpOM2. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | En Bloc |
| Donor Type | Lipid linked sugars |
| Donor Specificity | UndPP-Tetrasaccharide |
| Glycan Information | |
| Glycan transferred | Tetrasaccharide containing (HexNAc)2, Monosaccharide ( hexose) and N-acetyl-deoxyMonosaccharide ( hexose) |
| Method of Glycan Indentification | LC-MS, and MS-MS (CID and HCD) |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | PilA |
| ProGPdb ID | ProGP419 |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Harding, C.M., Nasr, M.A., Kinsella, R.L., Scott, N.E., Foster, L.J., Weber, B.S., Fiester, S.E., Actis, L.A., Tracy, E.N., Munson Jr, R.S. & Feldman, M. F. (2015). A cinetobacter strains carry two functional oligosaccharyltransferases, one devoted exclusively to type IV pilin, and the other one dedicated to O?glycosylation of multiple proteins. Molecular microbiology, 96(5), 1023-1041. |
| Authors | Harding, C.M., Nasr, M.A., Kinsella, R.L., Scott, N.E., Foster, L.J., Weber, B.S., Fiester, S.E., Actis, L.A., Tracy, E.N., Munson Jr, R.S. & Feldman, M. F. |
| Research groups | 1 Center for Microbial Pathogenesis, The Research Institute at Nationwide Childrens Hospital, Columbus, OH, USA.
2 Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA.
3 Biomedical Sciences Graduate Program, College of Medicine, The Ohio State University, Columbus, OH, USA.
4 Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
5 Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada.
6 Department of Microbiology, Miami University, Oxford, OH, USA |
| Corresponding Author | Feldman, M. F. |
| Contacts | Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2G2, Canada. |