ProGT89 (CcPglB)
| ProGT ID | ProGT89 (CcPglB) |
| Organism Information | |
| Organism Name | Campylobacter coli RM5611 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Campylobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 1365662 |
| Genome Information | |
| Gene Bank | NZ_CP007179 |
| Gene Information | |
| Gene Name | N218_16655 |
| NCBI Reference Sequence | AHK74917.1 |
| Protein information | |
| Protein Name | CcPglB |
| UniProtKB/ SwissProt ID | A0A059HT80 |
| UniProtKB Sequence | >tr|A0A059HT80|A0A059HT80_CAMJU Peptide-binding protein OS=Campylobacter jejuni K5 GN=N218_16655 PE=4 SV=1 MLKKEYFKNPTFILLAFIILAYVFSVLCRFYWIFWASEFNEYFFNNELMIISNDGYAFAE GARDMIAGFHQPNDLSYYGSSLSTLTYWFYKITPFSLESIFIYISTFLSSLVVIPLILIA NEYKRPLMGFVAALLASIANSYYNRTMSGYYDTDMLVIVLAMMIVFFMIRLILKKDLLSL ITLPLFVGIYLWWYPSSYTLNVALLGLFFIYTLVFHIKEKTLYMAIILASITLSNIAWFY QSAIIVILFSLFVLQNKRFSFALLGILGLATLVFLILSGGIDPILYQLKFYIFRSDESAN LAQGFMYFNVNQTIQEVESIDLSIFMQRISGSELVFFVSLIGFIFLVRKHKSMILALPML ALGFLALKSGLRFTIYAVPVLALGFGFLMSLLQERKQKNNNTYWWANIGVFIFTFLSLIP MFYHINNYKAPTVFSQNEATKLDELKKIAQREDYVVTWWDYGYPIRYYSDVKTLADGGKH LGKDNFFPSFVLSKDQVAAANMARLSVEYTEKSFYAPLNDILKNDLLQAMMKDYNQNNVD LFLASLSKPDFKINMPKTRDVYIYMPARMSLIFSTVASFSFVDLETGEINKPFTFSAAYP LDVKNGEIYLSNGIALSDDFRSFKINNSTISVNSIIEINSIKQGEYKITPIDDMAQFYIF YLKDSTIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLYDLVINARDAKVFKLKI |
| EMBL CDS | KDA34064.1 |
| Sequence length | 714 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | Lipid linked sugars |
| Donor Specificity | UndPP-Heptasaccharide |
| Glycan Information | |
| Glycan transferred | Heptasaccharide |
| Method of Glycan Indentification | LC-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | scFv13-R4 DQNAT |
| Acceptor Substrate name | scFv13-R4 AQNAT |
| Acceptor Substrate name | scFv13-R4 EQNAT |
| Acceptor Substrate name | TAMRA-GDQNATAF |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. (2015). Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5, 15237. |
| Authors | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. |
| Research groups | 1 School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853 USA.
2 Department of Microbiology, Cornell University, Ithaca, NY 14853 USA.
3 Comparative Biomedical Sciences, Cornell University, Ithaca, NY 14853 USA.
4 Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York |
| Corresponding Author | DeLisa, M. P. |
| Contacts | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |