ProGT91 (PglB)
| ProGT ID | ProGT91 (PglB) |
| Organism Information | |
| Organism Name | Campylobacter fetus subsp. fetus 82-40 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Campylobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 360106 |
| Genome Information | |
| Gene Bank | NC_008599.1 |
| EMBL | CP000487 |
| Gene Information | |
| Gene Name | CFF8240_1383 |
| NCBI Reference Sequence | ABK82109.1 |
| Protein information | |
| Protein Name | PglB |
| UniProtKB/ SwissProt ID | A0RQP1 |
| NCBI Ref Seq | WP_011732181.1 |
| UniProtKB Sequence | >tr|A0RQP1|A0RQP1_CAMFF General glycosylation pathway protein OS=Campylobacter fetus subsp. fetus (strain 82-40) GN=CFF8240_1383 PE=4 SV=1 MKNLGNFFMIFRSNTLFLIILAYIFSVICRFEWIYWASGYAEFFWNNQLMISTNDGYAYA EGARDMIAGFHQPNDLSFYGTPLSSITYLLVEITPFSLETIMVYLSVFLGGLVVIPIILM AREIGEIKAGFIAALLASIANSYYNRTMAGYYDTDMLIISLPIFTFWGIVRLYTKRDLKS FLIISFSMLIYSWWYPSSFSLNVAITLFFGLYTIIFDRKNILNYQAVLFMILAISGAEYF IRAILILALFVFIKFKPNLINTKVMISLYAAVLLFFIYFGGLNPIWFQLKFYIFRSVSDS DGVMFKYFNVNQTIMESGMIDFRFFAQRISGDIFVFLIALIGYVLLCFKYRIFLVTLPMI GLGFLSLKGGLRFTIYAVPMMAFGFGYALNWISKFIKIDKKHRKISLYAVTFFSFLPVIL LVLLQIYERIYLDEFKFLFPIFVVAYILFYFKFRREIATMVLFPLSLFALTPSLIHIYEY KPLTVFMNSEVKVLDKLKTIADPEDYVVAWWDYGYAIRYYSDTKTLIDGGKHLGRENFAV SWALSRPFISSANMARLEVEYTERGFEQKFNSNLNEMLKDYNASDVNGFLASLNDQNFTL PEKTRDIYYYLPDRMMGIFPVVTKFSRLDLVTAKEFRDAFFIVSTSFAQGGDGVVLSEGI TIANDGTKISFSGKTFRVNSYIETKYDKNMNLEKQIHHIDDNGGFYVVYMVDYGRFLIMD KEMFNSTYIQLFVLENYEGSDYEPIILDPSAKVYKLKR |
| EMBL CDS | ABK82109.1. |
| Sequence length | 758 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asp/Glu- Asn-Xaa-Ser/Thr |
| Donor Type | Lipid linked sugars |
| Donor Specificity | UndPP-Heptasaccharide |
| Glycan Information | |
| Glycan transferred | Heptasaccharide |
| Method of Glycan Indentification | LC-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | scFv13-R4 DQNAT |
| Acceptor Substrate name | TAMRA-GDQNATAF |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. (2015). Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5, 15237. |
| Authors | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. |
| Research groups | 1 School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853 USA.
2 Department of Microbiology, Cornell University, Ithaca, NY 14853 USA.
3 Comparative Biomedical Sciences, Cornell University, Ithaca, NY 14853 USA.
4 Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York. |
| Corresponding Author | DeLisa, M. P. |
| Contacts | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |