ProGT96 (DgPglB)
| ProGT ID | ProGT96 (DgPglB) |
| Organism Information | |
| Organism Name | Desulfovibrio gigas DSM 1382 |
| Clinical Implication | Non-pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : DeltaProteobacteria Orders : Desulfovibrionales Family : Desulfovibrionaceae Genus : Desulfovibrio Species : gigas Strain : DSM 1382 |
| Taxonomic ID (NCBI) | 1121448 |
| Genome Information | |
| Gene Bank | CP006585 |
| EMBL | CP006585 |
| Gene Information | |
| Gene Name | DGI_2311 |
| Protein information | |
| Protein Name | DgPglB |
| UniProtKB/ SwissProt ID | T2GBX6 |
| UniProtKB Sequence | >tr|T2GBX6|T2GBX6_DESGI Uncharacterized protein OS=Desulfovibrio gigas DSM 1382 = ATCC 19364 GN=DGI_2311 PE=4 SV=1 MSAPWNAVAPEQPARQELKLFLFVLGFTYALALAVHMHEIYGWQNPALVVDGEFIQGTHD SYHWLAGAKGVGEAVGTPLAVMAAGFASLTGMPLGLAGFVIPPLFAGLVGLVMAVWCRVL GWKEGAAAGGLVAGLLTVLSPGFYFRSRLGYYDTDMVTLLFPLLFTLGLAMAMGRWIRPS WRHREPLQEEPPQTIARVLAWLVLLGLLARFGAVWHGHILTFVKVLCGFSVCWGLAAGRP GARGRLMLGLAVFMASALVGWPGLACSLALWALAWKKPEMVSQWARAPWPGLAALVLLAV LGGVAQTTLQSATELLGAYLKPAADVTGSVNTTGVIYPGIGQSVIEVQNIALERVLERFH TSWLVAATGLLCFLGVLVLRPVALPLAIFLVMGLASVKLGSRLTMFGGAPMAIGLGMAVG WAGVALFRSGPGAGYKRFLLSCAATVVLGWPLVSFYISLPPTPVLSRQHCMALQSLKTSP PESKVWTWWDWGYPTHYYAERISFADGGRHYGHHIFPLGLVLTTPNPRLSAQLIKYSAQW NDEPWKAWDAMPRNEMLALLQGLGNATQPLPPFAAPRYVVATFENIRLAPWITYYGTWDM TTQQGLHGRLLEIRDAFRINYEKGEVQFTQREGVLPVASIDVLDRKERRSDVFPRNQGPH LVVHMAAQKYYLLDDQIYNSMMVQLLLEDPKKPRFGDYFSLVYDDFPDVRVFEVR |
| EMBL CDS | AGW14065.1 |
| Sequence length | 715 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Additional Information | 1) Among various homologs of PglBCj only PglBDg exhibited most relaxed specificity, glycosylate the Fc domain of human immunoglobulin G (IgG) at its native ‘QYNST’ sequon, the first example of native Fc glycosylation in Escherichia coli. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asp/Glu- Asn-Xaa-Ser/Thr |
| Donor Type | UndPP-Heptasaccharide |
| Donor Specificity | Lipid linked sugars |
| Glycan Information | |
| Glycan transferred | Heptasaccharide |
| Method of Glycan Indentification | LC-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | scFv13-R4 DQNAT |
| Acceptor Substrate name | scFv13-R4 AQNAT |
| Acceptor Substrate name | AcrA |
| ProGPdb ID | ProGP217 |
| Acceptor Substrate name | scFv13-R4 CQNAT |
| Acceptor Substrate name | scFv13-R4 EQNAT |
| Acceptor Substrate name | scFv13-R4 GQNAT |
| Acceptor Substrate name | scFv13-R4 HQNAT |
| Acceptor Substrate name | scFv13-R4 MQNAT |
| Acceptor Substrate name | scFv13-R4 NQNAT |
| Acceptor Substrate name | scFv13-R4 QQNAT |
| Acceptor Substrate name | scFv13-R4 SQNAT |
| Acceptor Substrate name | scFv13-R4 TQNAT |
| Acceptor Substrate name | scFv13-R4 VQNAT |
| Acceptor Substrate name | scFv13-R4 WQNAT |
| Acceptor Substrate name | scFv13-R4 YQNAT |
| Acceptor Substrate name | scFv13-R4 LQNAT |
| Acceptor Substrate name | scFv13-R4 FQNAT |
| Acceptor Substrate name | DYNST |
| Acceptor Substrate name | TAMRA-GAQNATAF |
| Acceptor Substrate name | TAMRA-GDQNATAF |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. and DeLisa, M.P., 2015. Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5(1), pp.1-13. |
| Corresponding Author | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |