ProGT97 (DvPglB)
| ProGT ID | ProGT97 (DvPglB) |
| Organism Information | |
| Organism Name | Desulfovibrio vulgaris subsp. vulgaris str. Hildenborough |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Desulfovibrionaceae Order: Desulfovibrionales Class: Deltaproteobacteria or Deltabacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 882 |
| Genome Information | |
| Gene Bank | AE017285 |
| EMBL | AE017285 |
| Gene Information | |
| Gene Name | DVU_1252 |
| Protein information | |
| Protein Name | DvPglB |
| UniProtKB/ SwissProt ID | Q72CN1 |
| NCBI Ref Seq | WP_010938548.1 |
| UniProtKB Sequence | >tr|Q72CN1|Q72CN1_DESVH Membrane protein, putative OS=Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303) GN=DVU_1252 PE=4 SV=1 MTRLRGTLSVCIDAFPAWTRPLALAFVVYALSFGMRMLEYPAWQNPEYMLDGEYLLATHD AYHWIAGAEGFEFGAGHPMSELVRLAAAFTGTTPAAVGFWLPPVMASLVAVGVFGWAAAL GGVEAGVCAGVLASLAPGFLARTLLGYCDTDLVTLLFPLLMGLGPAFWMARFMFTPLQVA RRLLARRTGTSDVVPDAVAVMMTSRGEADDDWHGGALSPRWLALLAGSGLVGSWGAEWHS LFPYLIRYDVVLLFGCICVFGRPGMRRALLVGGLAYALPMLVGAWGLLFALALGWGLHRA DARFMAFVRGRALPVAGWCAVAVLLFDPDVFRMFLNSIAGYVKRSGDPSPGAGGDDPLVY PSVAQSIIEVQDLSLSEVLSYFHPWLWVALAGLGGFVPLLLARPAAFFLLPLGVLSFLSV KLGGRMVMFGAPVLALGFALPMVWGVQRVLRHDLRTGWVRLALSAVLLVIVATPFVDLLP AMTQGPIINKRHAEALRHIRTATPEDSMVWIWWDWGYSAHHFAHRRTIADGASHGGPSLY VPAAVFSTANPRFAWQLIRYTAERGGIPGSVFEGMGGAQAQELVHRLGIEKMTFADAPRQ YLVVSYDMLRLGFWITNFGTWDFLSREGKGYAISSIPQQLSYSLDTGEVQVQGNTTGVLA STIDVFDEGQLERRDYIVRQGGSGNAIADYAARQRNIESRRNIHFLFNKVTGEKLVLDDR LYNTVMVQLLLCSPDDTRFAPYFKLIYDNVYARVYEVR |
| EMBL CDS | AAS95730.1 |
| Sequence length | 758 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| String | 882.DVU1252 |
| Additional Information | 1) It is able to transfer glycans to the acceptor sites and has relaxed substrate specificity towards negatively charged residue in the ?2 position relative to the asparagine as compare to C. jejuni PglB. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | Lipid linked sugars |
| Donor Specificity | UndPP-Heptasaccharide |
| Glycan Information | |
| Glycan transferred | Heptasaccharide |
| Method of Glycan Indentification | LC-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | scFv13-R4 LQNAT |
| Acceptor Substrate name | scFv13-R4 MQNAT |
| Acceptor Substrate name | scFv13-R4 QQNAT |
| Acceptor Substrate name | TAMRA-GDQNATAF |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. (2015). Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5, 15237. |
| Authors | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. |
| Research groups | 1 School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853 USA.
2 Department of Microbiology, Cornell University, Ithaca, NY 14853 USA.
3 Comparative Biomedical Sciences, Cornell University, Ithaca, NY 14853 USA.
4 Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York. |
| Corresponding Author | DeLisa, M. P. |
| Contacts | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |